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. 1975 Nov;16(5):1108–1116. doi: 10.1128/jvi.16.5.1108-1116.1975

Trichoplusia ni granulosis virus granulin: a phenol-soluble, phosphorylated protein.

M D Summers, G E Smith
PMCID: PMC355708  PMID: 1185849

Abstract

Trichoplusia ni granulosis virus granulin consists of one major polypeptide component with an estimated molecular weight of 28,000. The protein is phenol soluble, phosphorylated, and acidic. A protease activated by alkaline conditions is also associated with solubilized granulin preparations. If not properly inactivated, the protease will introduce extensive artifact into the protein giving rise to ambiguous and incorrect results as analyzed by SDS-polyacrylamide gel electrophoresis and peptide mapping. Procedures are documented for enzyme inactivation and the preparation of granulin in highly purified form for characterization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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