Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 Nov;16(5):1117–1123. doi: 10.1128/jvi.16.5.1117-1123.1975

Differential expression of helper viral structural polypeptides in cells transformed by clonal isolates of woolly monkey sarcoma virus.

S A Aaronson, J R Stephenson, S Hino, S R Tronick
PMCID: PMC355709  PMID: 171443

Abstract

Cell lines transformed by woolly monkey sarcoma virus (WSV) in the absence of infectious virus production were analyzed for the expression of woolly monkey helper viral p30, p12, and gp70 antigens. Several lines produced high levels of both p30 and p12, whereas gp70 was not detectable. One transformed clone expressed only p12, and in another cell line, none of the helper viral antigens were detected. The properties of each sarcoma virus bred true upon transmission, indicating that each variant represents a distinct genotype. The different cell lines were examined with respect to properties characteristic of the transformed state. The in vitro growth properties and oncogenicity of each WSV-transformed clone were indistinguishable, indicating that transformation by WSV occurs independently of the expression of at least three helper viral polypeptides.

Full text

PDF
1117

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A. Biologic characterization of mammalian cells transformed by a primate sarcoma virus. Virology. 1973 Apr;52(2):562–567. doi: 10.1016/0042-6822(73)90351-6. [DOI] [PubMed] [Google Scholar]
  2. Aaronson S. A. Chemical induction of focus-forming virus from nonproducer cells transformed by murine sarcoma virus. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3069–3072. doi: 10.1073/pnas.68.12.3069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aaronson S. A., Rowe S. P. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology. 1970 Sep;42(1):9–19. doi: 10.1016/0042-6822(70)90233-3. [DOI] [PubMed] [Google Scholar]
  4. Aaronson S. A., Todaro G. J. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science. 1968 Nov 29;162(3857):1024–1026. doi: 10.1126/science.162.3857.1024. [DOI] [PubMed] [Google Scholar]
  5. Aaronson S. A., Weaver C. A. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J Gen Virol. 1971 Nov;13(2):245–252. doi: 10.1099/0022-1317-13-2-245. [DOI] [PubMed] [Google Scholar]
  6. Bassin R. H., Phillips L. A., Kramer M. J., Haapala D. K., Peebles P. T., Nomura S., Fischinger P. J. Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1520–1524. doi: 10.1073/pnas.68.7.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bassin R. H., Tuttle N., Fischinger P. J. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int J Cancer. 1970 Jul 15;6(1):95–107. doi: 10.1002/ijc.2910060114. [DOI] [PubMed] [Google Scholar]
  8. Beemon K., Duesberg P., Vogt P. Evidence for crossing-over between avian tumor viruses based on analysis of viral RNAs. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4254–4258. doi: 10.1073/pnas.71.10.4254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Benveniste R. E., Scolnick E. M. RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA. Virology. 1973 Feb;51(2):370–382. doi: 10.1016/0042-6822(73)90436-4. [DOI] [PubMed] [Google Scholar]
  10. Bilello J. A., Strand M., August J. T. Murine sarcoma virus gene expression: transformants which express viral envelope glycoprotein in the absence of the major internal protein and infectious particles. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3234–3238. doi: 10.1073/pnas.71.8.3234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Eisenman R., Vogt V. M., Diggelmann H. Synthesis of avian RNA tumor virus structural proteins. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1067–1075. doi: 10.1101/sqb.1974.039.01.122. [DOI] [PubMed] [Google Scholar]
  12. Hanafusa H., Hanafusa T. Determining factor in the capacity of Rous sarcoma virus to induce tumors in mammals. Proc Natl Acad Sci U S A. 1966 Mar;55(3):532–538. doi: 10.1073/pnas.55.3.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hino S., Stephenson J. R., Aaronson S. A. Antigenic determinants of the 70,000 molecular weight glycoprotein of woolly monkey type C RNA virus. J Immunol. 1975 Oct;115(4):922–927. [PubMed] [Google Scholar]
  14. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1164–1169. doi: 10.1073/pnas.56.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Huu Duc-Nguyen, Rosenblum E. N., Zeigel R. F. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J Bacteriol. 1966 Oct;92(4):1133–1140. doi: 10.1128/jb.92.4.1133-1140.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kawakami T. G., Huff S. D., Buckley P. M., Dungworth D. L., Synder S. P., Gilden R. V. C-type virus associated with gibbon lymphosarcoma. Nat New Biol. 1972 Feb 9;235(58):170–171. doi: 10.1038/newbio235170a0. [DOI] [PubMed] [Google Scholar]
  17. Klement V., Nicolson M. O., Huebner R. J. Rescue of the genome of focus forming virus from rat non-productive lines by 5'-bromodeoxyruidine. Nat New Biol. 1971 Nov 3;234(44):12–14. doi: 10.1038/newbio234012a0. [DOI] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
  20. Naso R. B., Arcement L. J., Arlinghaus R. B. Biosynthesis of Rauscher leukemia viral proteins. Cell. 1975 Jan;4(1):31–36. doi: 10.1016/0092-8674(75)90130-0. [DOI] [PubMed] [Google Scholar]
  21. Parks W. P., Scolnick E. M., Noon M. C., Watson C. J., Kawakami T. G. Radioimmunoassay of mammalian type-C polypeptides. IV. Characterization of woolly monkey and gibbon viral antigens. Int J Cancer. 1973 Jul 15;12(1):129–137. doi: 10.1002/ijc.2910120114. [DOI] [PubMed] [Google Scholar]
  22. Scolnick E. M., Parks W. P. Isolation and characterization of a primate sarcoma virus: mechanism of rescue. Int J Cancer. 1973 Jul 15;12(1):138–147. doi: 10.1002/ijc.2910120115. [DOI] [PubMed] [Google Scholar]
  23. Scolnick E. M., Parks W., Kawakami T., Kohne D., Okabe H., Gilden R., Hatanaka M. Primate and murine type-C viral nucleic acid association kinetics: analysis of model systems and natural tissues. J Virol. 1974 Feb;13(2):363–369. doi: 10.1128/jvi.13.2.363-369.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Scolnick E. M., Rands E., Williams D., Parks W. P. Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus. J Virol. 1973 Sep;12(3):458–463. doi: 10.1128/jvi.12.3.458-463.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stephenson J. R., Aaronson S. A. Expression of endogenous RNA C-type virus group-specific antigens in mammalian cells. J Virol. 1973 Sep;12(3):564–569. doi: 10.1128/jvi.12.3.564-569.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stephenson J. R., Aaronson S. A. Murine sarcoma and leukemia viruses: genetic differences determined by RNA-DNA hybridization. Virology. 1971 Nov;46(2):480–484. doi: 10.1016/0042-6822(71)90048-1. [DOI] [PubMed] [Google Scholar]
  27. Stephenson J. R., Reynolds R. K., Aaronson S. A. Isolation of temperature-sensitive mutants of murine leukemia virus. Virology. 1972 Jun;48(3):749–756. doi: 10.1016/0042-6822(72)90158-4. [DOI] [PubMed] [Google Scholar]
  28. Theilen G. H., Gould D., Fowler M., Dungworth D. L. C-type virus in tumor tissue of a woolly monkey (Lagothrix spp.) with fibrosarcoma. J Natl Cancer Inst. 1971 Oct;47(4):881–889. [PubMed] [Google Scholar]
  29. Toyoshima K., Friis R. R., Vogt P. K. The reproductive and cell-transforming capacities of avian sarcoma virus B77: inactivation with UV light. Virology. 1970 Sep;42(1):163–170. doi: 10.1016/0042-6822(70)90249-7. [DOI] [PubMed] [Google Scholar]
  30. Tronick S. R., Stephenson J. R., Aaronson S. A. Comparative immunological studies of RNA C-type viruses: radioimmunoassay for a low molecular weight polypeptide of woolly monkey leukemia virus. Virology. 1974 Feb;57(2):347–356. doi: 10.1016/0042-6822(74)90174-3. [DOI] [PubMed] [Google Scholar]
  31. Tronick S. R., Stephenson J. R., Aaronson S. A. Immunological characterization of a low molecular weight polypeptide of murine leukemia virus. Virology. 1973 Jul;54(1):199–206. doi: 10.1016/0042-6822(73)90129-3. [DOI] [PubMed] [Google Scholar]
  32. Wolfe L. G., Smith R. K., Deinhardt F. Simian sarcoma virus, type 1 (Lagothrix): focus assay and demonstration of nontransforming associated virus. J Natl Cancer Inst. 1972 Jun;48(6):1905–1908. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES