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. 1975 Nov;16(5):1200–1207. doi: 10.1128/jvi.16.5.1200-1207.1975

Partial purification and properties of a bacteriophage T7 inhibitor of the host exonuclease V activity.

R Pacumbaba, M S Center
PMCID: PMC355719  PMID: 127051

Abstract

Infection of Escherichia coli with bacteriophage T7 results in an inhibition of the host exonuclease V (recB, C DNase) activity. This inhibition is not observed when cells are infected in the presence of chloramphenicol or with a gene 1 mutant. The protein responsible for the inhibition of exonuclease V has been partially purified from T7-infected cells. The protein which does not possess nuclease or ATPase activity can inhibit all nucleolytic activities associated with exonuclease V. The protein does not, however, inhibit the DNA-dependent ATPase activity associated with exonuclease V. The inhibitory protein has a molecular weight of about 12,000, as determined from sedimentation analysis in glycerol gradients.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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