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. 1975 Dec;16(6):1420–1425. doi: 10.1128/jvi.16.6.1420-1425.1975

Uukuniemi virus contains an RNA polymerase.

M Ranki, R F Pettersson
PMCID: PMC355750  PMID: 517

Abstract

An RNA-dependent RNA polymerase activity has been found associated with Uukuniemi virions. The enzyme activity is expressed only after disrupting the virions with the nonionic detergent Triton X-100 and is absolutely dependent on Mn2+, whereas Mg2+ is not required, a finding that distinguishes this polymerase from those of other enveloped minus-strand RNA viruses. Within the range pH 7.2 to 8.5 no distinct optimum was found. The optimum temperature was between 37 and 40 C. The reaction was not inhibited by actinomycin D, rifampin, or DNase, whereas RNase was completely inhibitory. The partially RNase-resistant product consisted of rather small-sized RNA, which contained sequences complementary to Uukuniemi virus RNA as shown by hybridization to the template L, M, and S RNA species of Uukuniemi virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. Expression of animal virus genomes. Bacteriol Rev. 1971 Sep;35(3):235–241. doi: 10.1128/br.35.3.235-241.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  4. Bishop D. H., Obijeski J. F., Simpson R. W. Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase. J Virol. 1971 Jul;8(1):66–73. doi: 10.1128/jvi.8.1.66-73.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bouloy M., Colbère F., Krams-Ozden S., Vialat P., Garapin A. C., Hannoun C., Lépine P. Activité RNA polymérasique à un bunyavirus (Lumbo) C R Acad Sci Hebd Seances Acad Sci D. 1975 Jan 13;280(2):213–215. [PubMed] [Google Scholar]
  6. Bouloy M., Krams-Ozden S., Horodniceanu F., Hannoun C. Three-segment RNA genome of Lumbo virus (Bunyavirus). Intervirology. 1973;2(3):173–180. doi: 10.1159/000149420. [DOI] [PubMed] [Google Scholar]
  7. Carter M. F., Biswal N., Rawls W. E. Polymerase activity of Pichinde virus. J Virol. 1974 Mar;13(3):577–583. doi: 10.1128/jvi.13.3.577-583.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Casals J. Antigenic characterization of tick-borne arboviruses not belonging to group B. Jpn J Med Sci Biol. 1967 Dec;20 (Suppl):119–129. [PubMed] [Google Scholar]
  9. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. McLerran C. J., Arlinghaus R. B. Structural components of a virus of the California encephalitis complex: LaCrosse virus. Virology. 1973 May;53(1):247–257. doi: 10.1016/0042-6822(73)90483-2. [DOI] [PubMed] [Google Scholar]
  12. Murphy F. A., Harrison A. K., Whitfield S. G. Bunyaviridae: morphologic and morphogenetic similarities of Bunyamwera serologic supergroup viruses and several other arthropod-borne viruses. Intervirology. 1973;1(4):297–316. doi: 10.1159/000148858. [DOI] [PubMed] [Google Scholar]
  13. OKER-BLOM N., SALMINEN A., BRUMMER-KORVENKONTIO M., KAEAERIAEINEN L., WECKSTROEM P. ISOLATION OF SOME VIRUSES OTHER THAN TYPICAL TICK-BORNE ENCEPHALITIS VIRUSES FROM IXODES RICINUS TICKS IN FINLAND. Ann Med Exp Biol Fenn. 1964;42:109–112. [PubMed] [Google Scholar]
  14. Pettersson R. F., von Bonsdorff C. H. Ribonucleoproteins of Uukuniemi virus are circular. J Virol. 1975 Feb;15(2):386–392. doi: 10.1128/jvi.15.2.386-392.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pettersson R., Käriäinen L. The ribonucleic acids of Uukuniemi virus, a noncubical tick-borne arbovirus. Virology. 1973 Dec;56(2):608–619. doi: 10.1016/0042-6822(73)90062-7. [DOI] [PubMed] [Google Scholar]
  16. Pettersson R., Käriäinen L., von Bonsdorff C. H., Oker-Blom N. Structural components of Uukuniemi virus, a noncubical tick-borne arbovirus. Virology. 1971 Dec;46(3):721–729. doi: 10.1016/0042-6822(71)90074-2. [DOI] [PubMed] [Google Scholar]
  17. Porterfield J. S., Casals J., Chumakov M. P., Gaidamovich S. Y., Hannoun C., Holmes I. H., Horzinek M. C., Mussgay M., Russell P. K. Bunyaviruses and bunyaviridae. Intervirology. 1974;2(4):270–272. doi: 10.1159/000149433. [DOI] [PubMed] [Google Scholar]
  18. Renkonen O., Käriäinen L., Pettersson R., Oker-Blom N. The phospholipid composition of Uukuniemi virus, a non-cubical tick-borne arbovirus. Virology. 1972 Dec;50(3):899–901. doi: 10.1016/0042-6822(72)90443-6. [DOI] [PubMed] [Google Scholar]
  19. Saikku P. Passerine birds in the ecology of Uukuniemi virus. Med Biol. 1974 Apr;52(2):98–103. [PubMed] [Google Scholar]
  20. Samso A., Bouloy M., Hannoun C. Présence de ribonucléoprotéines circulaires dans le virus Lumbo (Bunyavirus) C R Acad Sci Hebd Seances Acad Sci D. 1975 Feb 10;280(6):779–782. [PubMed] [Google Scholar]
  21. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
  22. von Bonsdorff C. H., Pettersson R. Surface structure of Uukuniemi virus. J Virol. 1975 Nov;16(5):1296–1307. doi: 10.1128/jvi.16.5.1296-1307.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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