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. 1975 Dec;16(6):1678–1682. doi: 10.1128/jvi.16.6.1678-1682.1975

Adenovirus assembly: self-assembly of partially digested hexons.

P A Boulanger
PMCID: PMC355776  PMID: 1202251

Abstract

Papainic hydrolysis of adenovirus type 2 hexon capsomers was apparently limited and induced self-aggregation of hexons in a preferred oligomeric species, hexon trimers, sedimenting at about 19X, and retaining their immunological properties. These hexon trimers were composed of two classes of trimer molecules with different electric charges. The relevance of this trimer aggregation phenomenon to the adenovirus assembly process is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. W., Baum P. R., Gesteland R. F. Processing of adenovirus 2-induced proteins. J Virol. 1973 Aug;12(2):241–252. doi: 10.1128/jvi.12.2.241-252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boulanger P. A., Flamencourt P., Biserte G. Isolation and comparative chemical study of structural proteins of the adenoviruses 2 and 5: hexon and fiber antigens. Eur J Biochem. 1969 Aug;10(1):116–131. doi: 10.1111/j.1432-1033.1969.tb00663.x. [DOI] [PubMed] [Google Scholar]
  3. Boulanger P. A., Puvion F. Adenovirus assembly. Cross-linking of adenovirus type-2 hexons in vitro. Eur J Biochem. 1974 Apr 16;43(3):465–470. doi: 10.1111/j.1432-1033.1974.tb03433.x. [DOI] [PubMed] [Google Scholar]
  4. Boulanger P. A., Puvion F. Large-scale preparation of soluble adenovirus hexon, penton and fiber antigens in highly purified form. Eur J Biochem. 1973 Nov 1;39(1):37–42. doi: 10.1111/j.1432-1033.1973.tb03100.x. [DOI] [PubMed] [Google Scholar]
  5. Crowther R. A., Franklin R. M. The structure of the groups of nine hexons from adenovirus. J Mol Biol. 1972 Jul 14;68(1):181–184. doi: 10.1016/0022-2836(72)90273-2. [DOI] [PubMed] [Google Scholar]
  6. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  7. Eiserling F. A., Dickson R. C. Assembly of viruses. Annu Rev Biochem. 1972;41:467–502. doi: 10.1146/annurev.bi.41.070172.002343. [DOI] [PubMed] [Google Scholar]
  8. Grütter M., Franklin R. M. Studies on the molecular weight of the adenovirus type 2 hexon and its subunit. J Mol Biol. 1974 Oct 15;89(1):163–178. doi: 10.1016/0022-2836(74)90168-5. [DOI] [PubMed] [Google Scholar]
  9. Jornvall H., Pettersson U., Philipson L. Structural studies of adenovirus type-2 hexon protein. Eur J Biochem. 1974 Oct 1;48(1):179–192. doi: 10.1111/j.1432-1033.1974.tb03755.x. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Liu T. Y., Chang Y. H. Hydrolysis of proteins with p-toluenesulfonic acid. Determination of tryptophan. J Biol Chem. 1971 May 10;246(9):2842–2848. [PubMed] [Google Scholar]
  12. Pereira H. G., Wrigley N. G. In vitro reconstitution, hexon bonding and handedness of incomplete adenovirus capsid. J Mol Biol. 1974 Jan 5;85(4):617–630. doi: 10.1016/0022-2836(74)90319-2. [DOI] [PubMed] [Google Scholar]
  13. Pettersson U., Philipson L., Höglund S. Structural proteins of adenoviruses. I. Purification and characterization of the adenovirus type 2 hexon antigen. Virology. 1967 Dec;33(4):575–590. doi: 10.1016/0042-6822(67)90057-8. [DOI] [PubMed] [Google Scholar]
  14. Pettersson U. Structural proteins of adenoviruses. VI. On the antigenic determinants of the hexon. Virology. 1971 Jan;43(1):123–136. doi: 10.1016/0042-6822(71)90230-3. [DOI] [PubMed] [Google Scholar]
  15. Philipson L., Pettersson U. Structure and function of virion proteins of adenoviruses. Prog Exp Tumor Res. 1973;18:1–55. doi: 10.1159/000393160. [DOI] [PubMed] [Google Scholar]
  16. Stinski M. F., Ginsberg H. S. Hexon peptides of type 2, 3, and 5 adenoviruses and their relationship to hexon structure. J Virol. 1975 Apr;15(4):898–905. doi: 10.1128/jvi.15.4.898-905.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Valentine R. C., Pereira H. G. Antigens and structure of the adenovirus. J Mol Biol. 1965 Aug;13(1):13–20. doi: 10.1016/s0022-2836(65)80076-6. [DOI] [PubMed] [Google Scholar]
  18. WILCOX W. C., GINSBERG H. S. STRUCTURE OF TYPE 5 ADENOVIRUS. I. ANTIGENIC RELATIONSHIP OF VIRUS-STRUCTURAL PROTEINS TO VIRUS-SPECIFIC SOLUBLE ANTIGENS FROM INFECTED CELLS. J Exp Med. 1963 Aug 1;118:295–306. doi: 10.1084/jem.118.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]

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