Table 1.
Limits of detection for indolamines and tyrosine in MEKC and CZE modes.
| Analyte | MEKC (nM)1 | CZE (nM)2 |
|---|---|---|
| 5-hydroxyindole-3-acetic acid, HIAA | 3.5 | 2.8 |
| 5-hydroxytryptophan, HTP | 5.4 | 1.6 |
| 5-hydroxtryptophol, HTOL | 2.6 | 6.7 |
| 5-methoxyindole-3-acetic acid, MIAA | 3.4 | 2.0 |
| 5-methoxytryptamine, MOT | 14 | 9.5 |
| 5-methoxytryptophan, MTrp | NA | 1.3 |
| 5-methoxytryptophol, MTOL | 3.5 | 3.0 |
| melatonin, MT | 14 | 2.8 |
| N-acetylserotonin, NAS | 5.1 | 3.7 |
| serotonin, 5-HT | 14 | 14 |
| tyrosine, Tyr | 39 | 17 |
| tryptophan, Trp | 6.0 | 1.6 |
| tryptophol, TOL | 2.5 | 2.6 |
1
MEKC conditions: 20 mM borate buffer, pH 8.8, + 50 mM SDS or 15 mM borate buffer, pH 8.8, + 37.7 mM SDS.
2
CZE conditions: 40 mM or 30 mM borate buffer, pH 8.8.