Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1971 Feb;7(2):214–220. doi: 10.1128/jvi.7.2.214-220.1971

Deoxyribonucleic Acid Polymerase Activity Associated with Strain MC29 Tumor Virus 1

G H Weber a, A A Kiessling a, G S Beaudreau a
PMCID: PMC356101  PMID: 5165089

Abstract

Chick embryo cells infected with strain MC29 tumor virus yielded progeny virus that contained detectable deoxyribonucleic acid (DNA) polymerase within the first 48 hr after infection. The noninfected culture fluids displayed no such enzyme activity when examined in an identical manner. Enzyme activity was greatly stimulated by adding DNA template to the reaction mixture and required all four deoxyribonucleoside triphosphates for full activity. When calf thymus DNA was used to direct synthesis, the DNA polymerase from the MC29 virus catalyzed the formation of DNA product having a higher buoyant density in CsCl. DNA product formed in the reaction directed by Micrococcus lysodeikticus DNA had the same buoyant density as the template DNA.

Full text

PDF
214

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEAUDREAU G. S., BECKER C. Virus of avian myeloblastosis. X. Photometric microdetermination of adenosinetriphosphatase activity. J Natl Cancer Inst. 1958 Feb;20(2):339–349. [PubMed] [Google Scholar]
  2. BONAR R. A., BEARD J. W. Virus of avian myeloblastosis. XII. Chemical constitution. J Natl Cancer Inst. 1959 Jul;23(1):183–197. [PubMed] [Google Scholar]
  3. Bolognesi D. P., Langlois A. J., Sverak L., Bonar R. A., Beard J. W. In vitro chick embryo cell response to strain MC29 avian leukosis virus. J Virol. 1968 Jun;2(6):576–586. doi: 10.1128/jvi.2.6.576-586.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Green M., Rokutanda M., Fujinaga K., Ray R. K., Rokutanda H., Gurgo C. Mechanism of carcinogenesis by RNA tumor viruses. I. An RNA-dependent DNA polymerase in murine sarcoma viruses. Proc Natl Acad Sci U S A. 1970 Sep;67(1):385–393. doi: 10.1073/pnas.67.1.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hatanaka M., Huebner R. J., Gilden R. V. DNA polymerase activity associated with RNA tumor viruses. Proc Natl Acad Sci U S A. 1970 Sep;67(1):143–147. doi: 10.1073/pnas.67.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Langlois A. J., Bolognesi D. P., Fritz R. B., Beard J. W. Strain MC29 avian leukosis virus release by chick embryo cells infected with the agent. Proc Soc Exp Biol Med. 1969 May;131(1):138–143. doi: 10.3181/00379727-131-33823. [DOI] [PubMed] [Google Scholar]
  7. Langlois A. J., Sankaran S., Hsiung P. H., Beard J. W. Massive direct conversion of chick embryo cells by strain MC29 avian leukosis virus. J Virol. 1967 Oct;1(5):1082–1084. doi: 10.1128/jvi.1.5.1082-1084.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mizutani S., Boettiger D., Temin H. M. A DNA-depenent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus. Nature. 1970 Oct 31;228(5270):424–427. doi: 10.1038/228424a0. [DOI] [PubMed] [Google Scholar]
  9. Ríman J., Beaudreau G. S. Viral DNA-dependent DNA polymerase and the properties of thymidine labelled material in virions of an oncogenic RNA virus. Nature. 1970 Oct 31;228(5270):427–430. doi: 10.1038/228427a0. [DOI] [PubMed] [Google Scholar]
  10. Ríman J., Sverak L., Langlois A. J., Bonar R. A., Beard J. W. Influence of toyocamycin on RNA synthesis in chick embryo cells noninfected and infected with strain MC29 avian leukosis virus. Cancer Res. 1969 Sep;29(9):1707–1716. [PubMed] [Google Scholar]
  11. Scolnick E. M., Aaronson S. A., Todaro G. J. DNA synthesis by RNA-containing tumor viruses. Proc Natl Acad Sci U S A. 1970 Oct;67(2):1034–1041. doi: 10.1073/pnas.67.2.1034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses. Nature. 1970 Aug 8;227(5258):563–567. doi: 10.1038/227563a0. [DOI] [PubMed] [Google Scholar]
  13. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. DNA-directed DNA polymerase activity in oncogenic RNA viruses. Nature. 1970 Sep 5;227(5262):1029–1031. doi: 10.1038/2271029a0. [DOI] [PubMed] [Google Scholar]
  14. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Trávnícek M., Watson K. Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses. Nature. 1970 Oct 31;228(5270):430–432. doi: 10.1038/228430a0. [DOI] [PubMed] [Google Scholar]
  15. Spiegelman S., Haruna I., Holland I. B., Beaudreau G., Mills D. The synthesis of a self-propagating and infectious nucleic acid with a purified enzyme. Proc Natl Acad Sci U S A. 1965 Sep;54(3):919–927. doi: 10.1073/pnas.54.3.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES