Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1971 Mar;7(3):343–351. doi: 10.1128/jvi.7.3.343-351.1971

Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses II. Relationship of Adenovirus 2 Deoxyribonucleic Acid and Simian Virus 40 Deoxyribonucleic Acid in the Ad2+ND1 Genome

Myron J Levin a,1, Clyde S Crumpacker a, Andrew M Lewis Jr a, Michael N Oxman a,1, Patrick H Henry a,2, Wallace P Rowe a
PMCID: PMC356124  PMID: 4323709

Abstract

A nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, Ad2+ND1, has been plaque-isolated from an Ad2-SV40 hybrid population. This virus, unlike the defective Ad-SV40 hybrid populations previously described, replicates without the aid of nonhybrid adenovirus helper. Consequently, the hybrid virus deoxyribonucleic acid (DNA) can be obtained free of nonhybrid adenovirus DNA. The DNA of the Ad2+ND1 virus was shown by ribonucleic acid (RNA)-DNA hybridization to consist of nucleotide sequences complementary to Ad2- and SV40-specific RNA. Techniques of equilibrium density and rate zonal centrifugation were employed to demonstrate that these Ad2 and SV40 nucleotide sequences were linked together in the same DNA molecules by alkali-resistant bonds. Calibration curves were established relating the amount of tritium-labeled SV40-specific RNA (prepared in vitro or in vivo) bound to given amounts of SV40 DNA in a hybridization reaction, and these curves were employed to determine the equivalent amount of SV40 DNA in the Ad2+ND1 molecule. From the results obtained, it was estimated that 1% of the Ad2+ND1 DNA consists of SV40 nucleotide sequences.

Full text

PDF
343

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  2. BLACK P. H., ROWE W. P. VIRAL STUDIES OF SV40 TUMORIGENESIS IN HAMSTERS. J Natl Cancer Inst. 1964 Jan;32:253–265. [PubMed] [Google Scholar]
  3. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baum S. G., Reich P. R., Hybner C. J., Rowe W. P., Weissman S. M. Biophysical evidence for linkage of adenovirus and SV40 DNA's in adenovirus 7-SV40 hybrid particles. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1509–1515. doi: 10.1073/pnas.56.5.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burnett J. P., Summers A. O., Harrington J. A., Dwyer A. C. Production of highly labeled adenoviruses. Appl Microbiol. 1968 Aug;16(8):1245–1250. doi: 10.1128/am.16.8.1245-1250.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CHAMBERLIN M., BERG P. Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli. Proc Natl Acad Sci U S A. 1962 Jan 15;48:81–94. doi: 10.1073/pnas.48.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CRAWFORD L. V., BLACK P. H. THE NUCLEIC ACID OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:388–392. doi: 10.1016/0042-6822(64)90176-x. [DOI] [PubMed] [Google Scholar]
  8. Crumpacker C. S., Henry P. H., Kakefuda T., Rowe W. P., Levin M. J., Lewis A. M., Jr Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. 3. Base composition, molecular weight, and conformation of the Ad2+ND genome. J Virol. 1971 Mar;7(3):352–358. doi: 10.1128/jvi.7.3.352-358.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GREEN M., PINA M. Similarity of DNAs isolated from tumor-inducing viruses of human and animal origin. Proc Natl Acad Sci U S A. 1963 Jul;50:44–46. doi: 10.1073/pnas.50.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  11. HOPPS H. E., BERNHEIM B. C., NISALAK A., TJIO J. H., SMADEL J. E. BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY. J Immunol. 1963 Sep;91:416–424. [PubMed] [Google Scholar]
  12. HUEBNER R. J., CHANOCK R. M., RUBIN B. A., CASEY M. J. INDUCTION BY ADENOVIRUS TYPE 7 OF TUMORS IN HAMSTERS HAVING THE ANTIGENIC CHARACTERISTICS OF SV40 VIRUS. Proc Natl Acad Sci U S A. 1964 Dec;52:1333–1340. doi: 10.1073/pnas.52.6.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KIRBY K. S. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID. Biochem J. 1965 Jul;96:266–269. doi: 10.1042/bj0960266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levin M. J., Oxman M. N., Diamandopoulos G. T., Levine A. S., Henry P. H., Enders J. F. Virus-specific nucleic acids in SV40-exposed hamster embryo cell lines: correlation with S and T antigens. Proc Natl Acad Sci U S A. 1969 Feb;62(2):589–596. doi: 10.1073/pnas.62.2.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Levine A. S., Oxman M. N., Henry P. H., Levin M. J., Diamandopoulos G. T., Enders J. F. Virus-specific deoxyribonucleic acid in simian virus 40-exposed hamster cells: correlation with S and T antigens. J Virol. 1970 Aug;6(2):199–207. doi: 10.1128/jvi.6.2.199-207.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lewis A. M., Jr, Baum S. G., Prigge K. O., Rowe W. P. Occurrence of adenovirus-SV40 hybrids among monkey kidney cell adapted strains of adenovirus. Proc Soc Exp Biol Med. 1966 May;122(1):214–218. doi: 10.3181/00379727-122-31093. [DOI] [PubMed] [Google Scholar]
  17. Lewis A. M., Jr, Levin M. J., Wiese W. H., Crumpacker C. S., Henry P. H. A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128–1135. doi: 10.1073/pnas.63.4.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lewis A. M., Jr, Prigge K. O., Rowe W. P. Studies of adenovirus-SV40 hybrid viruses. IV. An adenovirus type 2 strain carrying the infectious SV40 genome. Proc Natl Acad Sci U S A. 1966 Mar;55(3):526–531. doi: 10.1073/pnas.55.3.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lewis A. M., Jr, Rowe W. P. Studies on nondefective adenovirus-simian virus 40 hybrid viruses. I. A newly characterized simian virus 40 antigen induced by the Ad2+ND 1 virus. J Virol. 1971 Feb;7(2):189–197. doi: 10.1128/jvi.7.2.189-197.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Martin M. A., Axelrod D. SV40 gene activity during lytic infection and in a series of SV40 transformed mouse cells. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1203–1210. doi: 10.1073/pnas.64.4.1203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Meselson M., Stahl F. W., Vinograd J. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581–588. doi: 10.1073/pnas.43.7.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. O'CONOR G. T., RABSON A. S., BEREZESKY I. K., PAUL F. J. MIXED INFECTION WITH SIMIAN VIRUS 40 AND ADENOVIRUS 12. J Natl Cancer Inst. 1963 Oct;31:903–917. [PubMed] [Google Scholar]
  23. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. RAPP F., MELNICK J. L., BUTEL J. S., KITAHARA T. THE INCORPORATION OF SV40 MATERIAL INTO ADENOVIRUS 7 AS MEASURED BY INTRANUCLEAR SYNTHESIS OF SV40 TUMOR ANTIGEN. Proc Natl Acad Sci U S A. 1964 Dec;52:1348–1352. doi: 10.1073/pnas.52.6.1348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. ROWE W. P., BAUM S. G. EVIDENCE FOR A POSSIBLE GENETIC HYBRID BETWEEN ADENOVIRUS TYPE 7 AND SV40 VIRUSES. Proc Natl Acad Sci U S A. 1964 Dec;52:1340–1347. doi: 10.1073/pnas.52.6.1340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  27. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  28. Sauer G., Kidwai J. R. The transcription of the SV40 genome in productively infected and transformed cells. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1256–1263. doi: 10.1073/pnas.61.4.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schell K., Lane W. T., Casey M. J., Huebner R. J. Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization. Proc Natl Acad Sci U S A. 1966 Jan;55(1):81–88. doi: 10.1073/pnas.55.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. VINOGRAD J., MORRIS J., DAVIDSON N., DOVE W. F., Jr The bouyant behavior of viral and bacterial DNA in alkaline CsCl. Proc Natl Acad Sci U S A. 1963 Jan 15;49:12–17. doi: 10.1073/pnas.49.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wiese W. H., Lewis A. M., Jr, Rowe W. P. Equilibrium density gradient studies on simian virus 40-yielding variants of the adenovirus type 2-simian virus 40 hybrid population. J Virol. 1970 Apr;5(4):421–426. doi: 10.1128/jvi.5.4.421-426.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES