Abstract
The deoxyribonucleic acid (DNA) polymerase of Rous sarcoma virus synthesizes both single- and double-stranded DNA, utilizing the ribonucleic acid (RNA) of the viral genome as the initial template. Results of pulse-chase experiments indicate that the single-stranded DNA serves as unconserved template and precursor for the synthesis of double-stranded DNA. The latter reaction is apparently initiated in association with the viral RNA and may involve a partially double-stranded intermediate form.
Full text
PDF









Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
- Bishop J. M., Levinson W. E., Quintrell N., Sullivan D., Fanshier L., Jackson J. The low molecular weight RNAs of Rous sarcoma virus. I. The 4 S RNA. Virology. 1970 Sep;42(1):182–195. doi: 10.1016/0042-6822(70)90251-5. [DOI] [PubMed] [Google Scholar]
- Duesberg P. H., Canaani E. Complementarity between Rous sarcoma virus (RSV) RNA and the in vitro-synthesized DNA of the virus-associated DNA polymerase. Virology. 1970 Nov;42(3):783–788. doi: 10.1016/0042-6822(70)90325-9. [DOI] [PubMed] [Google Scholar]
- Englund P. T., Deutscher M. P., Jovin T. M., Kelly R. B., Cozzarelli N. R., Kornberg A. Structural and functional properties of Escherichia coli DNA polymerase. Cold Spring Harb Symp Quant Biol. 1968;33:1–9. doi: 10.1101/sqb.1968.033.01.005. [DOI] [PubMed] [Google Scholar]
- Fanshier L., Garapin A. C., McDonnell J., Faras A., Levinson W., Bishop J. M. Deoxyribonucleic acid polymerase associated with avian tumor viruses: secondary structure of the deoxyribonucleic acid product. J Virol. 1971 Jan;7(1):77–86. doi: 10.1128/jvi.7.1.77-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fujinaga K., Parsons J. T., Beard J. W., Beard D., Green M. Mechanism of carcinogenesis by RNA tumor viruses. 3. Formation of RNA, DNA complex and duplex DNA molecules by the DNA polymerase (s) of avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1432–1439. doi: 10.1073/pnas.67.3.1432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garapin A. C., Fanshier L., Leong J. A., Jackson J., Levinson W., Bishop J. M. Deoxyribonucleic acid polymerases of Rous sarcoma virus: kinetics of deoxyribonucleic acid synthesis and specificity of the products. J Virol. 1971 Feb;7(2):227–232. doi: 10.1128/jvi.7.2.227-232.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garapin A. C., McDonnell J. P., Levinson W., Quintrell N., Fanshier L., Bishop J. M. Deoxyribonucleic acid polymerase associated with Rous sarcoma virus and avian myeloblastosis virus: properties of the enzyme and its product. J Virol. 1970 Nov;6(5):589–598. doi: 10.1128/jvi.6.5.589-598.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manly K. F., Smoler D. F., Bromfeld E., Baltimore D. Forms of deoxyribonucleic acid produced by virions of the ribonucleic acid tumor viruses. J Virol. 1971 Jan;7(1):106–111. doi: 10.1128/jvi.7.1.106-111.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McDonnell J. P., Garapin A. C., Levinson W. E., Quintrell N., Fanshier L., Bishop J. M. DNA polymerases of Rous sarcoma virus: delineation of two reactions with actinomycin. Nature. 1970 Oct 31;228(5270):433–435. doi: 10.1038/228433a0. [DOI] [PubMed] [Google Scholar]
- Mizutani S., Boettiger D., Temin H. M. A DNA-depenent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus. Nature. 1970 Oct 31;228(5270):424–427. doi: 10.1038/228424a0. [DOI] [PubMed] [Google Scholar]
- Richardson C. C. Enzymes in DNA metabolism. Annu Rev Biochem. 1969;38:795–840. doi: 10.1146/annurev.bi.38.070169.004051. [DOI] [PubMed] [Google Scholar]
- Rokutanda M., Rokutanda H., Green M., Fujinaga K., Ray R. K., Gurgo C. Formation of viral RNA-DNA hybrid molecules by the DNA polymerase of sarcoma-leukaemia viruses. Nature. 1970 Sep 5;227(5262):1026–1028. doi: 10.1038/2271026a0. [DOI] [PubMed] [Google Scholar]
- Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses. Nature. 1970 Aug 8;227(5258):563–567. doi: 10.1038/227563a0. [DOI] [PubMed] [Google Scholar]
- Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. DNA-directed DNA polymerase activity in oncogenic RNA viruses. Nature. 1970 Sep 5;227(5262):1029–1031. doi: 10.1038/2271029a0. [DOI] [PubMed] [Google Scholar]
- Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Trávnícek M., Watson K. Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses. Nature. 1970 Oct 31;228(5270):430–432. doi: 10.1038/228430a0. [DOI] [PubMed] [Google Scholar]
- Stavis R. L., August J. T. The biochemistry of RNA bacteriophage replication. Annu Rev Biochem. 1970;39:527–560. doi: 10.1146/annurev.bi.39.070170.002523. [DOI] [PubMed] [Google Scholar]
- Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]