Abstract
Bacteriophage φX174 temperature-sensitive and nonsense mutations in eight cistrons were mapped by using two-, three-, and four-factor genetic crosses. The genetic map is circular with a total length of 24 × 10−4wt recombinants per progeny phage. The cistron order is D-E-F-G-H-A-B-C. High negative interference is seen, consistent with a small closed circular deoxyribonucleic acid molecule as a genome.
Full text
PDF









Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baker R., Tessman I. The circular genetic map of phage S13. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1438–1445. doi: 10.1073/pnas.58.4.1438. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benzer S. ON THE TOPOLOGY OF THE GENETIC FINE STRUCTURE. Proc Natl Acad Sci U S A. 1959 Nov;45(11):1607–1620. doi: 10.1073/pnas.45.11.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burgess A. B., Denhardt D. T. Studies on phiX174 proteins. I. Phage-specific proteins synthesized after infection of Escherichia coli. J Mol Biol. 1969 Sep 28;44(3):377–386. doi: 10.1016/0022-2836(69)90367-2. [DOI] [PubMed] [Google Scholar]
- Denhardt D. T., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. 3. Phage maturation and lysis after synchronized infection. J Mol Biol. 1965 Jul;12(3):641–646. doi: 10.1016/s0022-2836(65)80318-7. [DOI] [PubMed] [Google Scholar]
- Dowell C. E. Cold-sensitive mutants of bacteriophage phi-X-174. I. A mutant blocked in the eclipse function at low temperature. Proc Natl Acad Sci U S A. 1967 Sep;58(3):958–961. doi: 10.1073/pnas.58.3.958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FIERS W., SINSHEIMER R. L. The structure of the DNA of bacteriophage phi-X174. III. Ultracentrifugal evidence for a ring structure. J Mol Biol. 1962 Oct;5:424–434. doi: 10.1016/s0022-2836(62)80031-x. [DOI] [PubMed] [Google Scholar]
- Funk F. D., Sinsheimer R. L. Process of infection with bacteriophage phiX174. XXXV. Cistron 8. J Virol. 1970 Jul;6(1):12–19. doi: 10.1128/jvi.6.1.12-19.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gelfand D. H., Hayashi M. Electrophoretic characterization of phiX174-specific proteins. J Mol Biol. 1969 Sep 28;44(3):501–516. doi: 10.1016/0022-2836(69)90376-3. [DOI] [PubMed] [Google Scholar]
- HERSHEY A. D. The production of recombinants in phage crosses. Cold Spring Harb Symp Quant Biol. 1958;23:19–46. doi: 10.1101/sqb.1958.023.01.006. [DOI] [PubMed] [Google Scholar]
- Lindqvist B. H., Sinsheimer R. L. Process of infection with bacteriophage phi-X174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant. J Mol Biol. 1967 Aug 28;28(1):87–94. doi: 10.1016/s0022-2836(67)80079-2. [DOI] [PubMed] [Google Scholar]
- Mayol R. F., Sinsheimer R. L. Process of infection with bacteriophage phiX174. XXXVI. Measurement of virus-specific proteins during a normal cycle of infection. J Virol. 1970 Sep;6(3):310–319. doi: 10.1128/jvi.6.3.310-319.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PFEIFER D. Genetic recombination in bacteriophage phi-X 174. Nature. 1961 Feb 4;189:422–423. doi: 10.1038/189422a0. [DOI] [PubMed] [Google Scholar]
- PFEIFER D. [Genetic studies on the bacteriophage phi-X174. I. Development of a selective system and demonstration of a genetic recombination]. Z Vererbungsl. 1961;92:317–329. [PubMed] [Google Scholar]
- TESSMAN E. S. COMPLEMENTATION GROUPS IN PHAGE S13. Virology. 1965 Feb;25:303–321. doi: 10.1016/0042-6822(65)90208-4. [DOI] [PubMed] [Google Scholar]