Abstract
Infectious ribonucleic acids (IRNA) of Venezuelan equine encephalitis and Eastern equine encephalitis viruses were observed to form noninfectious complexes with a basic polyamino acid, poly-l-lysine. Original infectivity was recovered from the complexes by digestion of the polylysine with Pronase, and partial recovery was effected by treatment with sodium dodecyl sulfate. Infectivity could not be recovered from the complexes containing polylysine of 100,000 molecular weight by changes in ionic strength, pH, or by treatment with phenol, deoxycholate, or digitonin. Masking of infectivity by polylysine was demonstrated in vivo as well as by plaque assay in tissue culture. Poly-l-lysine preparations of high molecular weight (44,000 to 100,000) were more effective than low molecular weight (3,000) materials in masking infectivity of IRNA. When complexes, in which infectivity had been masked by low molecular weight polylysine, were suspended in 1 m NaCl, some infectivity was recovered. Complexes of polylysine-IRNA differed from control IRNA alone in (i) resistance to inactivation by ribonuclease, (ii) sedimentation patterns in sucrose gradient centrifugation, and (iii) stability of recoverable infectivity during different physical treatments.
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- COLON J. I., IDOINE J. B. FACTORS AFFECTING PLAQUE FORMATION BY THE INFECTIOUS RIBONUCLEIC ACID OF THE EQUINE ENCEPHALITIS VIRUSES. J Infect Dis. 1964 Feb;114:61–68. doi: 10.1093/infdis/114.1.61. [DOI] [PubMed] [Google Scholar]
- HARDY F. M. The growth of Venezuelan equine encephalomyelitis virus in various tissue cultures. Am J Hyg. 1959 Jul;70(1):21–27. doi: 10.1093/oxfordjournals.aje.a120061. [DOI] [PubMed] [Google Scholar]
- Higuchi S., Tsuboi M. Interaction of poly-L-lysine with nucleic acids. II. Poly(A+U), poly(A+2U), and rice dwarf virus RNA. Biopolymers. 1966 Sep;4(8):837–854. doi: 10.1002/bip.1966.360040804. [DOI] [PubMed] [Google Scholar]
- MANDEL B. THE EXTRACTION OF RIBONUCLEIC ACID FROM POLIOVIRUS BY TREATMENT WITH SODIUM DODECYL SULFATE. Virology. 1964 Mar;22:360–367. doi: 10.1016/0042-6822(64)90026-1. [DOI] [PubMed] [Google Scholar]
- MORA P. T., YOUNG B. G. Reversible blocking of T2 bacteriophage antiserum with polyelectrolytes. J Biol Chem. 1962 Jun;237:1870–1875. [PubMed] [Google Scholar]
- Matsuo K., Mitsui Y., Iitaka Y., Tsuboi M. Interaction of poly-L-lysine with nucleic acid. 3. An infrared and x-ray examination. J Mol Biol. 1968 Nov 28;38(1):129–132. doi: 10.1016/0022-2836(68)90133-2. [DOI] [PubMed] [Google Scholar]
- Norrell S. A., Costlow R. D. Effects of methylated albumin on infectious RNA: reversible infectivity loss and resistance to nuclease digestion. Biochem Biophys Res Commun. 1967 Feb 21;26(4):481–485. doi: 10.1016/0006-291x(67)90573-6. [DOI] [PubMed] [Google Scholar]
- Plescia O. J., Braun W. Nucleic acids as antigens. Adv Immunol. 1967;6:231–252. doi: 10.1016/s0065-2776(08)60523-4. [DOI] [PubMed] [Google Scholar]
- RICHTER A., WECKER E. The reaction of EEE virus preparations with sodium deoxycholate. Virology. 1963 Jun;20:263–268. doi: 10.1016/0042-6822(63)90114-4. [DOI] [PubMed] [Google Scholar]
- Sober H. A., Schlossman S. F., Yaron A., Latt SA RUSHIZKY G. W. Protein-nucleic acid interaction. I. Nuclease-resistant polylysine-ribonculeic acid complexes. Biochemistry. 1966 Nov;5(11):3608–3616. doi: 10.1021/bi00875a033. [DOI] [PubMed] [Google Scholar]
- Tsuboi M., Matsuo K., Ts'o P. O. Interaction of poly-L-lysine and nucleic acids. J Mol Biol. 1966 Jan;15(1):256–267. doi: 10.1016/s0022-2836(66)80225-5. [DOI] [PubMed] [Google Scholar]