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. 1971 May;7(5):603–611. doi: 10.1128/jvi.7.5.603-611.1971

Genetic Expression in Heterozygous Replicative Form Molecules of φX174

V Merriam a,1, L B Dumas a,2, R L Sinsheimer a
PMCID: PMC356169  PMID: 16789130

Abstract

Heterozygous replicative form molecules of bacteriophage φX174 deoxyribonucleic acid (DNA) have been constructed in vitro. These are composed of viral strands extracted from purified preparations of phage bearing ts mutations and complementary strands of either half length or full length synthesized with purified DNA polymerase, in vitro, on DNA from am3 phage. In infections with such heterozygous DNA, involving mutations in each of four different cistrons, phage with the genotype of the complementary strand comprised 1 to 20% of the total phage produced by a spheroplast population. From single-burst analysis of the progeny from DNA heterozygous in one cistron (B), it appears that those phage with the genotype of the complementary strand arise as major components in a small proportion of the infected cells rather than comprising a minor component in most cells. The implications of such a pattern of expression are discussed with respect to mechanisms of phage DNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  2. Denhardt D. T., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. 3. Phage maturation and lysis after synchronized infection. J Mol Biol. 1965 Jul;12(3):641–646. doi: 10.1016/s0022-2836(65)80318-7. [DOI] [PubMed] [Google Scholar]
  3. Doerfler W., Hogness D. S. Gene orientation in bacteriophage lambda as determined from the genetic activities of heteroduplex DNA formed in vitro. J Mol Biol. 1968 May 14;33(3):661–678. doi: 10.1016/0022-2836(68)90312-4. [DOI] [PubMed] [Google Scholar]
  4. Dressler D., Wolfson J. The rolling circle for phi X DNA replication. 3. Synthesis of supercoiled duplex rings. Proc Natl Acad Sci U S A. 1970 Sep;67(1):456–463. doi: 10.1073/pnas.67.1.456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dumas L. B., Darby G., Sinsheimer R. L. The replication of bacteriophage phi X174 DNA in vitro. Temperature effects on repair synthesis and displacement synthesis. Biochim Biophys Acta. 1971 Jan 28;228(2):407–422. [PubMed] [Google Scholar]
  6. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  7. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  8. Goulian M., Kornberg A. Enzymatic synthesis of DNA. 23. Synthesis of circular replicative form of phage phi-X174 DNA. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1723–1730. doi: 10.1073/pnas.58.4.1723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HAYASHI M., HAYASHI M. N., SPIEGELMAN S. RESTRICTION OF IN VIVO GENETIC TRANSCRIPTION TO ONE OF THE COMPLEMENTARY STRANDS OF DNA. Proc Natl Acad Sci U S A. 1963 Oct;50:664–672. doi: 10.1073/pnas.50.4.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hayashi M. N., Hayashi M. The stability of native DNA-RNA complexes during in vivo phiX-174 transcription. Proc Natl Acad Sci U S A. 1968 Nov;61(3):1107–1114. doi: 10.1073/pnas.61.3.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jansz H. S., van Rotterdam C., Cohen J. A. Genetic recombination of bacteriophage phi-X-174 in spheroplasts. Biochim Biophys Acta. 1966 May 19;119(2):276–288. [PubMed] [Google Scholar]
  12. Knippers R., Whalley J. M., Sinsheimer R. L. The process of infection with bacteriophage phiX174. XXX. Replication of double-stranded phiX DNA. Proc Natl Acad Sci U S A. 1969 Sep;64(1):275–282. doi: 10.1073/pnas.64.1.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Russo V. E., Stahl M. M., Stahl F. W. On the transfer of information from old to new chains of DNA duplexes in phage lambda: destruction of heterozygotes. Proc Natl Acad Sci U S A. 1970 Feb;65(2):363–367. doi: 10.1073/pnas.65.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sinsheimer R. L. "Contagium vivum fluidum", after seventy years. Proc K Ned Akad Wet C. 1970;73(3):307–316. [PubMed] [Google Scholar]
  15. Sinsheimer R. L., Knippers R., Komano T. Stages in the replication of bacteriophage phi X174 DNA in vivo. Cold Spring Harb Symp Quant Biol. 1968;33:443–447. doi: 10.1101/sqb.1968.033.01.051. [DOI] [PubMed] [Google Scholar]

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