Fig. 3.

GSCs lacking CycE arrest in a G1-like state showing a phenotype distinct from that of CycB-deficient GSCs. (A,A′) Mosaic germarium showing a GFP-negative CycE^AR95 clone. CycB (red) is shown in greyscale in A′. GFP (green), wild-type control cells; 1B1 (blue), fusomes; LamC (blue), cap cell nuclear envelopes. (B-E) CycE^AR95 (B-D) or CycB² (E) mosaic germaria showing GFP-negative clones. Arrows indicate unusual fusome morphology (see supplementary material Fig. S1 for comparison). GFP (green), wild-type control cells; 1B1 (red), fusomes; LamC (red), cap cell nuclear envelopes; DAPI (blue), nuclei. Solid outline indicates wild-type GSCs; dashed outlines indicate mutant GSCs. Scale bar: 5 μm.