Embryonic expression of CAP protein. (A-D) Wild-type (A,C) and dCAP49e (B,D) embryos stained with anti-CAP and 22C10 antibody (which labels all sensory neurons). (A,B) Wild-type embryos show CAP staining in chordotonal organs (arrowheads), dorsal vessels (thin arrows) and gut (thick arrows); this staining is absent in CAP mutants. (C,D) Higher magnification image of CAP localization in chordotonal organ scolopale cells (arrows) in wild type, but not dCAP49e,embryos. (E,F) Wild-type and dCAP49e embryos immunostained with anti-CAP and anti-βPS integrin; CAP colocalizes with βPS integrin at MASs (arrows). Scale bars: 60 μm in A,B; 20 μm in C-F.