Abstract
Sodium dodecyl sulfate was found to aggregate virions from the Sabin Lsc2ab strain of poliovirus type 1. Aggregation was prevented by high ionic strength buffers. A procedure is described for the rapid purification of the virus through the use of sarkozyl.
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Selected References
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- Cooper P. D., Summers D. F., Maizel J. V. Evidence for ambiguity in the posttranslational cleavage of poliovirus proteins. Virology. 1970 Jul;41(3):408–418. doi: 10.1016/0042-6822(70)90161-3. [DOI] [PubMed] [Google Scholar]
- Fiszman M. Y., Bucchini D., Girard M., Lwoff A. Inhibition of poliovirus RNA synthesis by supraoptimal temperatures. J Gen Virol. 1970 Feb;6(2):293–304. doi: 10.1099/0022-1317-6-2-293. [DOI] [PubMed] [Google Scholar]
- Girard M., Marty L., Suarez F. Capsid proteins of Simian virus 40. Biochem Biophys Res Commun. 1970 Jul 13;40(1):97–102. doi: 10.1016/0006-291x(70)91051-x. [DOI] [PubMed] [Google Scholar]
- Granboulan N., Girard M. Molecular weight of poliovirus ribonucleic acid. J Virol. 1969 Oct;4(4):475–479. doi: 10.1128/jvi.4.4.475-479.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANDEL B. THE EXTRACTION OF RIBONUCLEIC ACID FROM POLIOVIRUS BY TREATMENT WITH SODIUM DODECYL SULFATE. Virology. 1964 Mar;22:360–367. doi: 10.1016/0042-6822(64)90026-1. [DOI] [PubMed] [Google Scholar]
- Maizel J. V., Jr, Summers D. F. Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells. Virology. 1968 Sep;36(1):48–54. doi: 10.1016/0042-6822(68)90115-3. [DOI] [PubMed] [Google Scholar]
- PENMAN S., BECKER Y., DARNELL J. E. A CYTOPLASMIC STRUCTURE INVOLVED IN THE SYNTHESIS AND ASSEMBLY OF POLIOVIRUS COMPONENTS. J Mol Biol. 1964 Apr;8:541–555. doi: 10.1016/s0022-2836(64)80010-3. [DOI] [PubMed] [Google Scholar]
- Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]