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. 1971 Jun;7(6):776–782. doi: 10.1128/jvi.7.6.776-782.1971

Synthesis of the Major Bacteriophage f1 Coat Protein

Henry Smilowitz 1, Harvey Lodish 1, Phillips W Robbins 1
PMCID: PMC356197  PMID: 4935446

Abstract

A method which allows one to follow the synthesis of the major f1 coat protein in normal, unirradiated f1-infected cells is reported. The N-terminal tryptic peptide of this protein, labeled with 14C-lysine, has a negative charge at pH 4.5 and is readily separated from the contaminating peptides of host cell proteins. This technique was used to study several aspects of the synthesis of the major f1 coat protein in infected cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Frank H., Day L. A. Electron microscopic observations on fd bacteriophage, its alkali denaturation products and its DNA. Virology. 1970 Sep;42(1):144–154. doi: 10.1016/0042-6822(70)90247-3. [DOI] [PubMed] [Google Scholar]
  2. HOFFMANN BERLING H., MAZE R. RELEASE OF MALE-SPECIFIC BACTERIOPHAGES FROM SURVIVING HOST BACTERIA. Virology. 1964 Mar;22:305–313. doi: 10.1016/0042-6822(64)90021-2. [DOI] [PubMed] [Google Scholar]
  3. HOFSCHNEIDER P. H., PREUSS A. M 13 BACTERIOPHAGE LIBERATION FROM INTACT BACTERIA AS REVEALED BY ELECTRON MICROSCOPY. J Mol Biol. 1963 Oct;7:450–451. doi: 10.1016/s0022-2836(63)80038-8. [DOI] [PubMed] [Google Scholar]
  4. Henry T. J., Pratt D. The proteins of bacteriophage M13. Proc Natl Acad Sci U S A. 1969 Mar;62(3):800–807. doi: 10.1073/pnas.62.3.800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Pratt D., Erdahl W. S. Genetic control of bacteriophage M13 DNA synthesis. J Mol Biol. 1968 Oct 14;37(1):181–200. doi: 10.1016/0022-2836(68)90082-x. [DOI] [PubMed] [Google Scholar]
  6. Pratt D., Tzagoloff H., Beaudoin J. Conditional lethal mutants of the small filamentous coliphage M13. II. Two genes for coat proteins. Virology. 1969 Sep;39(1):42–53. doi: 10.1016/0042-6822(69)90346-8. [DOI] [PubMed] [Google Scholar]
  7. Pratt D., Tzagoloff H., Erdahl W. S. Conditional lethal mutants of the small filamentous coliphage M13. I. Isolation, complementation, cell killing, time of cistron action. Virology. 1966 Nov;30(3):397–410. doi: 10.1016/0042-6822(66)90118-8. [DOI] [PubMed] [Google Scholar]
  8. Rossomando E. F., Zinder N. D. Studies on the bacteriophage fl. I. Alkali-induced disassembly of the phage into DNA and protein. J Mol Biol. 1968 Sep 28;36(3):387–399. doi: 10.1016/0022-2836(68)90163-0. [DOI] [PubMed] [Google Scholar]
  9. SALIVAR W. O., TZAGOLOFF H., PRATT D. SOME PHYSICAL-CHEMICAL AND BIOLOGICAL PROPERTIES OF THE ROD-SHAPED COLIPHAGE M13. Virology. 1964 Nov;24:359–371. doi: 10.1016/0042-6822(64)90173-4. [DOI] [PubMed] [Google Scholar]
  10. Stegen U., Hofschneider P. H. Replication of the single-stranded DNA bacteriophage M13: absence of intracellular phages. J Mol Biol. 1970 Mar 14;48(2):361–364. doi: 10.1016/0022-2836(70)90168-3. [DOI] [PubMed] [Google Scholar]
  11. Trenkner E. Pool sizes of fd bacteriophage components in infected bacterial cells. Virology. 1970 Jan;40(1):18–22. doi: 10.1016/0042-6822(70)90374-0. [DOI] [PubMed] [Google Scholar]

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