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. 1971 Jun;7(6):813–820. doi: 10.1128/jvi.7.6.813-820.1971

Transfer of Thymidine Kinase to Thymidine Kinaseless L Cells by Infection with Ultraviolet-Irradiated Herpes Simplex Virus

William Munyon a, Edmundo Kraiselburd a,1, Daniel Davis a,2, Judith Mann a
PMCID: PMC356201  PMID: 4327589

Abstract

L cells lacking thymidine kinase (TK) activity (Ltk cells) have been stably transformed to a TK-positive phenotype by infection with ultraviolet-irradiated herpes simplex virus (HSV-UV). The highest frequency of the Ltk to Ltk+ transformation observed in these experiments was approximately 10−3, whereas no measurable transformation was observed (less than 10−8) in the absence of HSV-UV infection. Cell lines of HSV-transformed Ltk+ cell lines contain 7 to 24 times as much TK activity as do the parental Ltk cells, and they have been maintained in culture for a period exceeding 8 months. The kinetics of thermal inactivation of the TK activity derived from an Ltk+ HSV-transformed cell line and the TK activity from Ltk cells lytically infected with infectious HSV are similar. Both of these TK activities are much more thermolabile than the TK activity present in wild-type L cells. A mutant strain of HSV which does not induce TK activity during lytic infection does not cause the Ltk to Ltk+ transformation. These data suggest that either an HSV TK gene has been transferred to Ltk cells or that an HSV gene product has caused the expression of a previously repressed cellular enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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