Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1971 Jul;8(1):7–16. doi: 10.1128/jvi.8.1.7-16.1971

Initial Stage of Transformation of Permissive Cells by Simian Virus 40: Development of Resistance to Productive Infection

E C Hahn 1, G Sauer 1
PMCID: PMC356208  PMID: 4105906

Abstract

A quantitative assay has been used to determine the conditions leading to acquisition of resistance of permissive cells to lytic infection. The number of cell colonies surviving infection depends on the occurrence of several cell divisions after infection. High yields of resistant colonies were obtained when infected, confluent cultures were released from contact inhibition 10 to 14 hr after infection. Infection of actively growing cells produced similar results, but halting further division by seeding these growing cells on confluent monolayers prevented the development of colonies. Colony formation was a direct function of multiplicities lower than 5. An inverse killing response was observed with higher multiplicities, yet colonies were produced at a multiplicity of infection as high as 50. Brief exposure of input simian virus 40 to ultraviolet light stimulated colony formation. Irradiation of the virus for longer periods of time led to reduction of colony formation at a rate slower than the rate of inactivation of viral infectivity. It was concluded that resistance is induced by simian virus 40 and that this alteration represents one of the earliest detectable characteristics of the transformation of permissive cells.

Full text

PDF
7

Images in this article

9Image
on p.9
14Image
on p.14

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A. Effect of ultraviolet irradiation on the survival of simian virus 40 functions in human and mouse cells. J Virol. 1970 Oct;6(4):393–399. doi: 10.1128/jvi.6.4.393-399.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BADER J. P. Production of interferon by chick embryo cells exposed to Rous sarcoma virus. Virology. 1962 Apr;16:436–443. doi: 10.1016/0042-6822(62)90224-6. [DOI] [PubMed] [Google Scholar]
  3. Barbanti-Brodano G., Swetly P., Koprowski H. Superinfection of simian virus 40-transformed permissive cells with simian virus 40. J Virol. 1970 Nov;6(5):644–651. doi: 10.1128/jvi.6.5.644-651.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Basilico C., Di Mayorca G. Radiation target size of the lytic and the transforming ability of polyoma virus. Proc Natl Acad Sci U S A. 1965 Jul;54(1):125–127. doi: 10.1073/pnas.54.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benjamin T. L. Relative target sizes for the inactivation of the transforming and reproductive abilities of polyoma virus. Proc Natl Acad Sci U S A. 1965 Jul;54(1):121–124. doi: 10.1073/pnas.54.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carp R. I., Gilden R. V. A comparison of the replication cycles of simian virus 40 in human diploid and African green monkey kidney cells. Virology. 1966 Jan;28(1):150–162. doi: 10.1016/0042-6822(66)90316-3. [DOI] [PubMed] [Google Scholar]
  7. Cassingena R., Tournier P. Mise en évidence d'un "répresseur" spéfique dans des cellules d'espèces différentes transformées par le virus SV 40. C R Acad Sci Hebd Seances Acad Sci D. 1968 Dec 16;267(25):2251–2254. [PubMed] [Google Scholar]
  8. FOGH J., FOGH H. A METHOD FOR DIRECT DEMONSTRATION OF PLEUROPNEUMONIA-LIKE ORGANISMS IN CULTURED CELLS. Proc Soc Exp Biol Med. 1964 Dec;117:899–901. doi: 10.3181/00379727-117-29731. [DOI] [PubMed] [Google Scholar]
  9. Fernandes M. V., Moorhead P. S. Transformation of African green monkey kidney cultures infected with simian vacuolating virus (SV40). Tex Rep Biol Med. 1965 Jun;23(Suppl):242–258. [PubMed] [Google Scholar]
  10. Girardi A. J. Prevention of SV40 virus oncogenesis in hamsters. I. Tumor resistance induced by human cells transformed by SV40. Proc Natl Acad Sci U S A. 1965 Aug;54(2):445–451. doi: 10.1073/pnas.54.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. JENSEN F. C., GIRARDI A. J., GILDEN R. V., KOPROWSKI H. INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS. Proc Natl Acad Sci U S A. 1964 Jul;52:53–59. doi: 10.1073/pnas.52.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jensen F. C., Koprowski H. Absence of repressor in SV40-transformed cells. Virology. 1969 Apr;37(4):687–690. doi: 10.1016/0042-6822(69)90290-6. [DOI] [PubMed] [Google Scholar]
  13. Kit S., Kurimura T., Salvi M. L., Dubbs D. R. Activation of infectious SV40 synthesis in transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1239–1246. doi: 10.1073/pnas.60.4.1239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Koprowski H., Jensen F. C., Steplewski Z. Activation of production of infectious tumor virus SV40 in heterokaryon cultures. Proc Natl Acad Sci U S A. 1967 Jul;58(1):127–133. doi: 10.1073/pnas.58.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sauer G., Hahn E. C. The interaction of SV40 with SV40-transformed and non-transformed monkey kidey cells. Z Krebsforsch. 1970;74(1):40–47. doi: 10.1007/BF00524678. [DOI] [PubMed] [Google Scholar]
  16. Sauer G., Hahn E. C. The superinfection of abortively infected monkey kidney cells with SV40. Brief report. Arch Gesamte Virusforsch. 1970;30(2):267–270. doi: 10.1007/BF01250200. [DOI] [PubMed] [Google Scholar]
  17. Sauer G., Koprowski H., Defendi V. The genetic heterogeneity of simian virus 40. Proc Natl Acad Sci U S A. 1967 Aug;58(2):599–606. doi: 10.1073/pnas.58.2.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stoker M. Abortive transformation by polyoma virus. Nature. 1968 Apr 20;218(5138):234–238. doi: 10.1038/218234a0. [DOI] [PubMed] [Google Scholar]
  19. Swetly P., Brodano G. B., Knowles B., Koprowski H. Response of simian virus 40-transformed cell lines and cell hybrids to superinfection with simian virus 40 and its deoxyribonucleic acid. J Virol. 1969 Oct;4(4):348–355. doi: 10.1128/jvi.4.4.348-355.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Todaro G. J., Green H. Cell growth and the initiation of transformation by SV40. Proc Natl Acad Sci U S A. 1966 Feb;55(2):302–308. doi: 10.1073/pnas.55.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Uchida S., Watanabe S., Kato M. Incomplete growth of simian virus 40 in African green monkey kidney culture induced by serial undiluted passages. Virology. 1966 Jan;28(1):135–141. doi: 10.1016/0042-6822(66)90314-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES