Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1971 Jul;8(1):66–73. doi: 10.1128/jvi.8.1.66-73.1971

Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase I. Optimal Conditions for In Vitro Activity of the Ribonucleic Acid-Dependent Ribonucleic Acid Polymerase

David H L Bishop 1,2, John F Obijeski 1,2, Robert W Simpson 1,2
PMCID: PMC356215  PMID: 4328417

Abstract

In vitro reaction conditions have been determined for the maximal synthesis of product ribonucleic acid by the influenza (WSN) virion ribonucleic acid polymerase. The reaction requires the presence of all four triphosphates, Mg2+ and Mn2+ ions, monovalent cations, nonionic detergent, and ribonucleoside triphosphates at concentrations above certain threshold values. The optimum pH for the reaction is around 8.0 to 8.2 and the kinetics of product synthesis are linear through at least 6 hr when incubated at 31 to 33 C.

Full text

PDF
66

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRESLER A. E. The preparation of labeled uridine 5-triphosphate by the action of mono- and diphosphouridine kinases from Escherichia coli. Biochim Biophys Acta. 1962 Jul 9;61:29–33. doi: 10.1016/0926-6550(62)90025-7. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop D. H. Complete transcription by the transcriptase of vesicular stomatitis virus. J Virol. 1971 Apr;7(4):486–490. doi: 10.1128/jvi.7.4.486-490.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blair C. D., Duesberg P. H. Myxovirus ribonucleic acids. Annu Rev Microbiol. 1970;24:539–574. doi: 10.1146/annurev.mi.24.100170.002543. [DOI] [PubMed] [Google Scholar]
  5. Brown F., Martin S. J., Cartwright B., Crick J. The ribonucleic acids of the infective and interfering components of vesicular stomatitis virus. J Gen Virol. 1967 Oct;1(4):479–486. doi: 10.1099/0022-1317-1-4-479. [DOI] [PubMed] [Google Scholar]
  6. Chow N. L., Simpson R. W. RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses. Proc Natl Acad Sci U S A. 1971 Apr;68(4):752–756. doi: 10.1073/pnas.68.4.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HARUNA I., NOZU K., OHTAKA Y., SPIEGELMAN S. AN RNA "REPLICASE" INDUCED BY AND SELECTIVE FOR A VIRAL RNA: ISOLATION AND PROPERTIES. Proc Natl Acad Sci U S A. 1963 Nov;50:905–911. doi: 10.1073/pnas.50.5.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEHMAN I. R., BESSMAN M. J., SIMMS E. S., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. J Biol Chem. 1958 Jul;233(1):163–170. [PubMed] [Google Scholar]
  9. Pons M. W., Hirst G. K. Polyacrylamide gel electrophoresis of influenza virus RNA. Virology. 1968 Feb;34(2):385–388. doi: 10.1016/0042-6822(68)90257-2. [DOI] [PubMed] [Google Scholar]
  10. Roy P., Bishop D. H. Nucleoside triphosphate phosphotransferase. A new enzyme activity of oncogenic and non-oncogenic "budding" viruses. Biochim Biophys Acta. 1971 Apr 14;235(1):191–206. doi: 10.1016/0005-2744(71)90047-7. [DOI] [PubMed] [Google Scholar]
  11. SIMPSON R. W., HIRST G. K. Genetic recombination among influenza viruses. I. Cross reactivation of plaque-forming capacity as a method for selecting recombinants from the progeny of crosses between influenza A strains. Virology. 1961 Dec;15:436–451. doi: 10.1016/0042-6822(61)90111-8. [DOI] [PubMed] [Google Scholar]
  12. Scholtissek C., Rott R. Effect of temperature on the multiplication of an Influenza virus. J Gen Virol. 1969 Sep;5(2):283–290. doi: 10.1099/0022-1317-5-2-283. [DOI] [PubMed] [Google Scholar]
  13. Simpson R. W., Hirst G. K. Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation. Virology. 1968 May;35(1):41–49. doi: 10.1016/0042-6822(68)90303-6. [DOI] [PubMed] [Google Scholar]
  14. Symons R. H. Modified procedure for the synthesis of 32P-labelled ribonucleoside 5'-monophosphates of high specific activity. Biochim Biophys Acta. 1968 Feb 26;155(2):609–610. doi: 10.1016/0005-2787(68)90205-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES