Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1971 Jul;8(1):74–80. doi: 10.1128/jvi.8.1.74-80.1971

Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase II. Nature of the In Vitro Polymerase Product

David H L Bishop 1,2, John F Obijeski 1,2, Robert W Simpson 1,2
PMCID: PMC356216  PMID: 5106347

Abstract

The properties of the ribonucleic acid (RNA) synthesized by the influenza (WSN) virion polymerase have been investigated. Most of the product RNA is synthesized in association with virion RNA species from which it can be released by heat treatment as single-stranded, ribonuclease-sensitive polynucleotides (average molecular weight, 2-hr sample, about 105 daltons). At least 95% of the product is complementary to the viral RNA species. On the basis of the molar ratio of the RNA species isolated from a 3H-uridine-labeled virus preparation, it was calculated that the WSN genome consists of seven pieces of RNA with a sum molecular weight of about 5 × 106 daltons.

Full text

PDF
74

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  3. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  4. Bishop D. H. Complete transcription by the transcriptase of vesicular stomatitis virus. J Virol. 1971 Apr;7(4):486–490. doi: 10.1128/jvi.7.4.486-490.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bishop D. H., Obijeski J. F., Simpson R. W. Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase. J Virol. 1971 Jul;8(1):66–73. doi: 10.1128/jvi.8.1.66-73.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Blair C. D., Duesberg P. H. Myxovirus ribonucleic acids. Annu Rev Microbiol. 1970;24:539–574. doi: 10.1146/annurev.mi.24.100170.002543. [DOI] [PubMed] [Google Scholar]
  7. Chow N. L., Simpson R. W. RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses. Proc Natl Acad Sci U S A. 1971 Apr;68(4):752–756. doi: 10.1073/pnas.68.4.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Compans R. W., Klenk H. D., Caliguiri L. A., Choppin P. W. Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology. 1970 Dec;42(4):880–889. doi: 10.1016/0042-6822(70)90337-5. [DOI] [PubMed] [Google Scholar]
  9. Duesberg P. H. The RNA of influenza virus. Proc Natl Acad Sci U S A. 1968 Mar;59(3):930–937. doi: 10.1073/pnas.59.3.930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lerner R. A., Hodge L. D. Nonpermissive infections of mammalian cells: synthesis of inluenza virus genome in HeLa cells. Proc Natl Acad Sci U S A. 1969 Oct;64(2):544–551. doi: 10.1073/pnas.64.2.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lin F. H., Thormar H. Ribonucleic acid-dependent deoxyribonucleic acid polymerase in visna virus. J Virol. 1970 Nov;6(5):702–704. doi: 10.1128/jvi.6.5.702-704.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Loening U. E. Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol. 1968 Dec;38(3):355–365. doi: 10.1016/0022-2836(68)90391-4. [DOI] [PubMed] [Google Scholar]
  14. Pons M. W., Hirst G. K. Polyacrylamide gel electrophoresis of influenza virus RNA. Virology. 1968 Feb;34(2):385–388. doi: 10.1016/0042-6822(68)90257-2. [DOI] [PubMed] [Google Scholar]
  15. Roy P., Bishop D. H. Isolation and properties of poliovirus minus strand ribonucleic acid. J Virol. 1970 Nov;6(5):604–609. doi: 10.1128/jvi.6.5.604-609.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schulze I. T. The structure of influenza virus. I. The polypeptides of the virion. Virology. 1970 Dec;42(4):890–904. doi: 10.1016/0042-6822(70)90338-7. [DOI] [PubMed] [Google Scholar]
  17. Shatkin A. J., Sipe J. D. RNA polymerase activity in purified reoviruses. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462–1469. doi: 10.1073/pnas.61.4.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES