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. 1971 Sep;8(3):293–302. doi: 10.1128/jvi.8.3.293-302.1971

System of Double Infection Between Vaccinia Virus and Mengovirus

Celia E Freda a, Clayton A Buck a,1
PMCID: PMC356242  PMID: 4330362

Abstract

When L cells are simultaneously infected with vaccinia virus and mengovirus, double interference in the replication of both viruses is observed. Superinfection of vaccinia virus-infected cells by mengovirus during the first 5 hr of infection reduces vaccinia virus yields to between 1 and 3% of controls. The yields of mengovirus are reduced to between 1 and 16% of controls, depending upon the time of superinfection. The replication of vaccinia deoxyribonucleic acid is not inhibited by mengovirus; it is only delayed. On the other hand, vaccinia multiplication severely hinders the replication of mengovirus ribonucleic acid. The double-infected system, at early times, synthesizes proteins that resemble those synthesized in the vaccinia virus-infected cells. Later in infection, however, the pattern is switched to proteins synthesized by mengovirus-infected cells. Possible mechanisms for this double interference in multiplication are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aubertin A. M., Guir J., Kirn A. The inhibition of vaccinia virus DNA synthesis in KB cells infected with frog virus 3. J Gen Virol. 1970 Aug;8(2):105–111. doi: 10.1099/0022-1317-8-2-105. [DOI] [PubMed] [Google Scholar]
  2. Buck C. A., Granger G. A., Taylor M. W., Holland J. J. Efficient, inefficient, and abortive infection of different mammalian cells by small RNA viruses. Virology. 1967 Sep;33(1):36–46. doi: 10.1016/0042-6822(67)90091-8. [DOI] [PubMed] [Google Scholar]
  3. CORDS C. E., HOLLAND J. J. INTERFERENCE BETWEEN ENTEROVIRUSES AND CONDITIONS EFFECTING ITS REVERSAL. Virology. 1964 Feb;22:226–234. doi: 10.1016/0042-6822(64)90007-8. [DOI] [PubMed] [Google Scholar]
  4. Choppin P. W., Holmes K. V. Replication of SV5 RNA and the effects of superinfection with poliovirus. Virology. 1967 Nov;33(3):442–451. doi: 10.1016/0042-6822(67)90119-5. [DOI] [PubMed] [Google Scholar]
  5. Dales S., Silverberg H. Controlled double infection with unrelated animal viruses. Virology. 1968 Mar;34(3):531–543. doi: 10.1016/0042-6822(68)90072-x. [DOI] [PubMed] [Google Scholar]
  6. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  7. Giorno R., Kates J. R. Mechanism of inhibition of vaccinia virus replication in adenovirus-infected HeLa cells. J Virol. 1971 Feb;7(2):208–213. doi: 10.1128/jvi.7.2.208-213.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hattman S., Hofschneider P. H. Influence of T4 on the formation of RNA phage-specific polyribosomes and polymerase. J Mol Biol. 1968 Aug 14;35(3):513–522. doi: 10.1016/s0022-2836(68)80011-7. [DOI] [PubMed] [Google Scholar]
  9. Hattman S., Hofschneider P. H. Interference of bacteriophage T4 in the reproduction of RNA-phage M12. J Mol Biol. 1967 Oct 14;29(1):173–190. doi: 10.1016/0022-2836(67)90189-1. [DOI] [PubMed] [Google Scholar]
  10. Holland J. J., Kiehn E. D. Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses. Proc Natl Acad Sci U S A. 1968 Jul;60(3):1015–1022. doi: 10.1073/pnas.60.3.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huppert J., Blum-Emerique L., Breugnon M. M. Mixed infection of Escherichia coli by RNA and DNA bacteriphages. I. Inhibition of RNA phage multiplication by superinfection with DNA phages. Virology. 1967 Oct;33(2):307–316. doi: 10.1016/0042-6822(67)90149-3. [DOI] [PubMed] [Google Scholar]
  12. JOKLIK W. K. The preparation and characteristics of highly purified radioactively labelled poxvirus. Biochim Biophys Acta. 1962 Aug 20;61:290–301. doi: 10.1016/0926-6550(62)90091-9. [DOI] [PubMed] [Google Scholar]
  13. Joklik W. K., Merigan T. C. Concerning the mechanism of action of interferon. Proc Natl Acad Sci U S A. 1966 Aug;56(2):558–565. doi: 10.1073/pnas.56.2.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kates J. R., McAuslan B. R. Messenger RNA synthesis by a "coated" viral genome. Proc Natl Acad Sci U S A. 1967 Feb;57(2):314–320. doi: 10.1073/pnas.57.2.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kates J. R., McAuslan B. R. Poxvirus DNA-dependent RNA polymerase. Proc Natl Acad Sci U S A. 1967 Jul;58(1):134–141. doi: 10.1073/pnas.58.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Katz E., Moss B. Formation of a vaccinia virus structural polypeptide from a higher molecular weight precursor: inhibition by rifampicin. Proc Natl Acad Sci U S A. 1970 Jul;66(3):677–684. doi: 10.1073/pnas.66.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Klem E. B., Hsu W. T., Weiss S. B. The selective inhibition of protein initiation by T4 phage-induced factors. Proc Natl Acad Sci U S A. 1970 Oct;67(2):696–701. doi: 10.1073/pnas.67.2.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Magee W. E., Miller O. V. Initiation of vaccinia virus infection in actinomycin D-pretreated cells. J Virol. 1968 Jul;2(7):678–685. doi: 10.1128/jvi.2.7.678-685.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McCormick W., Penman S. Replication of mengovirus in HeLa cells preinfected with nonreplicating poliovirus. J Virol. 1968 Sep;2(9):859–864. doi: 10.1128/jvi.2.9.859-864.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moss B., Rosenblum E. N., Katz E., Grimley P. M. Rifampicin: a specific inhibitor of vaccinia virus assembly. Nature. 1969 Dec 27;224(5226):1280–1284. doi: 10.1038/2241280a0. [DOI] [PubMed] [Google Scholar]
  21. Oda K. I., Joklik W. K. Hybridization and sedimentation studies on "early" and "late" vaccinia messenger RNA. J Mol Biol. 1967 Aug 14;27(3):395–419. doi: 10.1016/0022-2836(67)90047-2. [DOI] [PubMed] [Google Scholar]
  22. Plagemann P. G., Swim H. E. Symposium on replication of viral nucleic acids. 3. Replication of mengovirus ribonucleic acid. Bacteriol Rev. 1966 Jun;30(2):288–308. doi: 10.1128/br.30.2.288-308.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SALZMAN N. P., SHATKIN A. J., SEBRING E. D. Viral protein and DNA synthesis in vaccinia virus-infected HeLacell cultures. Virology. 1963 Apr;19:542–550. doi: 10.1016/0042-6822(63)90049-7. [DOI] [PubMed] [Google Scholar]
  24. Sebring E. D., Salzman N. P. Metabolic properties of early and late vaccinia virus messenger ribonucleic acid. J Virol. 1967 Jun;1(3):550–558. doi: 10.1128/jvi.1.3.550-558.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yarosh E., Levinthal C. Exclusion of RNA bacteriophages and interference with their RNA replication by bacteriophage T4. J Mol Biol. 1967 Dec 14;30(2):329–348. [PubMed] [Google Scholar]

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