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. 1972 Feb;9(2):223–230. doi: 10.1128/jvi.9.2.223-230.1972

Cell-Dependent Differences in the Production of Infectious Herpes Simplex Virus at a Supraoptimal Temperature

Norman A Crouch 1, Fred Rapp 1
PMCID: PMC356286  PMID: 4335516

Abstract

The replication of herpes simplex virus (HSV) was compared in rabbit and hamster cells at optimal and supraoptimal temperatures. Replication occurred in cells of either species at 33 C, but the total infectious virus yield was routinely about 10-fold greater in rabbit cells than in hamster cells. At 39 C, this difference was exaggerated to greater than 100,000-fold. Whereas infectious virus was produced and plaques formed in rabbit kidney cell monolayers at the higher temperature, neither developed in those derived from hamster embryos. Elevating the temperature from 33 C to 39 C at various time intervals after exposure of the cultures to virus revealed that production of infectious virus in hamster cells was completely heat-sensitive up to 6 hr after infection. Specific viral antigens and viral deoxyribonucleic acid (DNA) were synthesized in both rabbit and hamster cell cultures. In addition, cellular DNA synthesis was depressed and cytopathic effects occurred in both cell systems. These cytopathic effects were not observed in cell cultures treated with HSV previously inactivated with ultraviolet light. Compared with parallel cultures at 33 C, the amount of viral DNA synthesized at 39 C was greatly reduced in both systems. In hamster cells, the reduction was twofold greater than in rabbit cells. This cell-dependent thermal inhibition of HSV replication in hamster cells did not occur with vaccinia virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen G. H., Vaughan R. K., Lawrence W. C. Deoxyribonucleic acid synthesis in synchronized mammalian KB cells infected with herpes simplex virus. J Virol. 1971 Jun;7(6):783–791. doi: 10.1128/jvi.7.6.783-791.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Duff R., Rapp F. Oncogenic transformation of hamster cells after exposure to herpes simplex virus type 2. Nat New Biol. 1971 Sep 8;233(36):48–50. doi: 10.1038/newbio233048a0. [DOI] [PubMed] [Google Scholar]
  3. Duff R., Rapp F. Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2. J Virol. 1971 Oct;8(4):469–477. doi: 10.1128/jvi.8.4.469-477.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FARNHAM A. E., NEWTON A. A. The effect of some environmental factors on herpes virus grown in HeLa cells. Virology. 1959 Apr;7(4):449–461. doi: 10.1016/0042-6822(59)90073-x. [DOI] [PubMed] [Google Scholar]
  5. Gharpure M. A. A heat-sensitive cellular function required for the replication of DNA viruses but not RNA viruses. Virology. 1965 Nov;27(3):308–319. doi: 10.1016/0042-6822(65)90110-8. [DOI] [PubMed] [Google Scholar]
  6. HOGGAN M. D., ROIZMAN B. The effect of the temperature of incubation on the formation and release of herpes simplex virus in infected FL cells. Virology. 1959 Aug;8:508–524. doi: 10.1016/0042-6822(59)90052-2. [DOI] [PubMed] [Google Scholar]
  7. KAPLAN A. S. A study of the herpes simplex virus-rabbit kidney cell system by the plaque technique. Virology. 1957 Dec;4(3):435–457. doi: 10.1016/0042-6822(57)90078-8. [DOI] [PubMed] [Google Scholar]
  8. KAPLAN A. S., BEN-PORAT T. The pattern of viral and cellular DNA synthesis in pseudorabies virus-infected cells in the logarithmic phase of growth. Virology. 1963 Feb;19:205–214. doi: 10.1016/0042-6822(63)90010-2. [DOI] [PubMed] [Google Scholar]
  9. Kieff E. D., Bachenheimer S. L., Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. doi: 10.1128/jvi.8.2.125-132.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Longson M. A temperature marker test for the differentiation of strains of Herpesvirus hominis. Ann Inst Pasteur (Paris) 1971 May;120(5):699–708. [PubMed] [Google Scholar]
  11. Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Guest B. A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. II. Women with cervical cancer. Am J Epidemiol. 1970 Jun;91(6):547–552. doi: 10.1093/oxfordjournals.aje.a121166. [DOI] [PubMed] [Google Scholar]
  12. Plummer G., Waner J. L., Phuangsab A., Goodheart C. R. Type 1 and type 2 herpes simplex viruses: serological and biological differences. J Virol. 1970 Jan;5(1):51–59. doi: 10.1128/jvi.5.1.51-59.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rawls W. E., Tompkins W. A., Melnick J. L. The association of herpesvirus type 2 and carcinoma of the uterine cervix. Am J Epidemiol. 1969 May;89(5):547–554. doi: 10.1093/oxfordjournals.aje.a120967. [DOI] [PubMed] [Google Scholar]
  14. Royston I., Aurelian L. The association of genital herpesvirus with cervical atypia and carcinoma in situ. Am J Epidemiol. 1970 Jun;91(6):531–538. doi: 10.1093/oxfordjournals.aje.a121164. [DOI] [PubMed] [Google Scholar]
  15. STICH H. F., HSU T. C., RAPP F. VIRUSES AND MAMMALIAN CHROMOSOMES. I. LOCALIZATION OF CHROMOSOME ABERRATIONS AFTER INFECTION WITH HERPES SIMPLEX VIRUS. Virology. 1964 Apr;22:439–445. doi: 10.1016/0042-6822(64)90064-9. [DOI] [PubMed] [Google Scholar]
  16. Sydiskis R. J., Roizman B. Polysomes and protein synthesis in cells infected with a DNA virus. Science. 1966 Jul 1;153(3731):76–78. doi: 10.1126/science.153.3731.76. [DOI] [PubMed] [Google Scholar]
  17. WHEELER C. E. The effect of temperature upon the production of herpes simplex virus in tissue culture. J Immunol. 1958 Aug;81(2):98–106. [PubMed] [Google Scholar]
  18. Wagner E. K., Roizman B. Ribonucleic acid synthesis in cells infected with herpes simplex virus. I. Patterns of ribonucleic acid synthesis in productively infected cells. J Virol. 1969 Jul;4(1):36–46. doi: 10.1128/jvi.4.1.36-46.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yoshino K., Taniguchi S., Takeuchi H. Suppression of growth of herpes simplex virus in chick embryo fibroblasts at 40 degrees C. Jpn J Microbiol. 1968 Mar;12(1):97–102. doi: 10.1111/j.1348-0421.1968.tb00371.x. [DOI] [PubMed] [Google Scholar]

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