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. 1972 Feb;9(2):251–257. doi: 10.1128/jvi.9.2.251-257.1972

Mechanism of Antiviral Action of Acetone on Rabbitpox Virus Replication

V I Chernos 1, B A Libshits 1, E Yakobson 1, Y Z Ghendon 1
PMCID: PMC356290  PMID: 5062678

Abstract

Acetone added to the maintenance medium in a 1% concentration reduces the yield of infectious rabbitpox virus in L-cell monolayer cultures by 90 to 97%. This concentration of the inhibitor is not toxic for cells. It was established that there is an inhibitor-sensitive stage late in the infectious cycle. Acetone exerted no significant influence on production of early viral messenger ribonucleic acid, formation of polyribosomes, deoxyribonucleic acid (DNA) replication, or protein synthesis. In the presence of acetone, the assembly of so-called “acetone particles” occurred. These particles are similar to normal virions by morphological and sedimentation properties but are slightly different from them in buoyant density. The amount of virus-specific DNA and the optical density of the “acetone particles” are the same as those of normal virions despite a 10- to 25-fold difference in the infectivity of the preparations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basilico C., Joklik W. K. Studies on a temperature-sensitive mutant of vaccinia virus strain WR. Virology. 1968 Dec;36(4):668–677. doi: 10.1016/0042-6822(68)90198-0. [DOI] [PubMed] [Google Scholar]
  2. Dales S. Effects of streptovitacin A on the initial events in the replication of vaccinia and reovirus. Proc Natl Acad Sci U S A. 1965 Aug;54(2):462–468. doi: 10.1073/pnas.54.2.462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dales S. Effects of streptovitacin A on the initial events in the replication of vaccinia and reovirus. Proc Natl Acad Sci U S A. 1965 Aug;54(2):462–468. doi: 10.1073/pnas.54.2.462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ghendon Y., Samoilova G. Antiviral effect of acetone. J Gen Virol. 1968 Sep;3(2):271–273. doi: 10.1099/0022-1317-3-2-271. [DOI] [PubMed] [Google Scholar]
  5. Grimley P. M., Rosenblum E. N., Mims S. J., Moss B. Interruption by Rifampin of an early stage in vaccinia virus morphogenesis: accumulation of membranes which are precursors of virus envelopes. J Virol. 1970 Oct;6(4):519–533. doi: 10.1128/jvi.6.4.519-533.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JOKLIK W. K., BECKER Y. THE REPLICATION AND COATING OF VACCINIA DNA. J Mol Biol. 1964 Dec;10:452–474. doi: 10.1016/s0022-2836(64)80066-8. [DOI] [PubMed] [Google Scholar]
  7. JOKLIK W. K. The purification fo four strains of poxvirus. Virology. 1962 Sep;18:9–18. doi: 10.1016/0042-6822(62)90172-1. [DOI] [PubMed] [Google Scholar]
  8. Katz E., Moss B. Formation of a vaccinia virus structural polypeptide from a higher molecular weight precursor: inhibition by rifampicin. Proc Natl Acad Sci U S A. 1970 Jul;66(3):677–684. doi: 10.1073/pnas.66.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mcauslan B. R. Rifampicin inhibition of vaccinia replication. Biochem Biophys Res Commun. 1969 Oct 8;37(2):289–295. doi: 10.1016/0006-291x(69)90733-5. [DOI] [PubMed] [Google Scholar]
  10. Moss B. Inhibition of HeLa cell protein synthesis by the vaccinia virion. J Virol. 1968 Oct;2(10):1028–1037. doi: 10.1128/jvi.2.10.1028-1037.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moss B., Katz E., Rosenblum E. N. Vaccinia virus directed RNA and protein synthesis in the presence of rifampicin. Biochem Biophys Res Commun. 1969 Aug 22;36(5):858–865. doi: 10.1016/0006-291x(69)90688-3. [DOI] [PubMed] [Google Scholar]
  12. Moss B., Rosenblum E. N., Katz E., Grimley P. M. Rifampicin: a specific inhibitor of vaccinia virus assembly. Nature. 1969 Dec 27;224(5226):1280–1284. doi: 10.1038/2241280a0. [DOI] [PubMed] [Google Scholar]
  13. Mss B., Filler R. Irreversible effects of cycloheximide during the early period of vaccinia virus replicaon. J Virol. 1970 Feb;5(2):99–108. doi: 10.1128/jvi.5.2.99-108.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nagaya A., Pogo B. G., Dales S. Biogenesis of vaccinia: separation of early stages from maturation by means of rifampicin. Virology. 1970 Apr;40(4):1039–1051. doi: 10.1016/0042-6822(70)90150-9. [DOI] [PubMed] [Google Scholar]
  15. Quintrell N. A., McAuslan B. R. Inhibition of Poxvirus replication by streptovaricin. J Virol. 1970 Oct;6(4):485–491. doi: 10.1128/jvi.6.4.485-491.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rosenkranz H. S., Rose H. M., Morgan C., Hsu K. C. The effect of hydroxyurea on virus development. II. Vaccinia virus. Virology. 1966 Apr;28(4):510–519. doi: 10.1016/0042-6822(66)90235-2. [DOI] [PubMed] [Google Scholar]
  17. SALZMAN N. P., SHATKIN A. J., SEBRING E. D. THE SYNTHESIS OF A DNA-LIKE RNA IN THE CYTOPLASM OF HELA CELLS INFECTED WITH VACCINIA VIRUS. J Mol Biol. 1964 Mar;8:405–416. doi: 10.1016/s0022-2836(64)80204-7. [DOI] [PubMed] [Google Scholar]
  18. SHATKIN A. J. ACTINOMYCIN D AND VACCINIA VIRUS INFECTION OF HELA CELLS. Nature. 1963 Jul 27;199:357–358. doi: 10.1038/199357a0. [DOI] [PubMed] [Google Scholar]
  19. Salzman N. P., Sebring E. D. Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. J Virol. 1967 Feb;1(1):16–23. doi: 10.1128/jvi.1.1.16-23.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tan K. B., McAuslan B. R. Effect of rifampicin on poxvirus protein synthesis. J Virol. 1970 Sep;6(3):326–332. doi: 10.1128/jvi.6.3.326-332.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WESTWOOD J. C., PHIPPS P. H., BOULTER E. A. The titration of vaccinia virus on the chorioallantoic membrane of the developing chick embryo. J Hyg (Lond) 1957 Mar;55(1):123–139. doi: 10.1017/s0022172400061301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wacker A., Chandra P., Mildner P., Feller H. Photodynamic action of acetone on the template activity of nucleic acids. Biophysik. 1968 May 15;4(4):283–288. doi: 10.1007/BF01195038. [DOI] [PubMed] [Google Scholar]

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