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. 1972 Feb;9(2):280–285. doi: 10.1128/jvi.9.2.280-285.1972

Transduction of Gal+ by Coliphage T1 II. Role of λ Transcription Control in the Efficiency of Transduction

Henry Drexler 1
PMCID: PMC356294  PMID: 5014932

Abstract

One of the crossovers leading to the transduction of Escherichia coli W3350 or one of its derivatives for the Gal+ marker by T1 grown on donors lysogenic for λ must occur to the right of the galactose operon. The location of this crossover determined both the rate of transduction and the λ genes which control the transduction by (λ-gal)T1. When the crossover occurred either to the left of gene N or the right of the cI gene, it was affected in a positive fashion by the gene product of the N+ gene. When the crossover occurred either to the left of gene N or between N and gene Q, its efficiency was not significantly affected by the expression of Q+. However, crossovers to the right of gene Q were greatly stimulated by the presence of a functioning Q gene on the chromosome of (λ-gal)T1 hybrid. The repressor made by the cI+ gene of λ blocked efficient transduction. Therefore, the control of efficient transduction by (λ-gal)T1 is the same as the control of transcription of λ. It is concluded that the increased efficiency of transduction by (λ-gal)T1 in nonimmune recipients is not caused by any particular product of a gene but rather by the process of transcription itself.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTTIN G., JACOB F., MONOD J. [Constituent synthesis of galactokinase following the development of lambda bacteriophages in Escherichia coli K 12]. C R Hebd Seances Acad Sci. 1960 Mar 28;250:2471–2473. [PubMed] [Google Scholar]
  2. Bovre K., Szybalski W. Patterns of convergent and overlapping transcription within the b2 region of coliphage lambda. Virology. 1969 Aug;38(4):614–626. doi: 10.1016/0042-6822(69)90181-0. [DOI] [PubMed] [Google Scholar]
  3. Court D., Sato K. Studies of novel transducing variants of lambda: dispensability of genes N and Q. Virology. 1969 Oct;39(2):348–352. doi: 10.1016/0042-6822(69)90060-9. [DOI] [PubMed] [Google Scholar]
  4. Dove W. F. Action of the lambda chromosome. I. Control of functions late in bacteriophage development. J Mol Biol. 1966 Aug;19(1):187–201. doi: 10.1016/s0022-2836(66)80060-8. [DOI] [PubMed] [Google Scholar]
  5. Drexler H. Transduction by bacteriophage T1. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1083–1088. doi: 10.1073/pnas.66.4.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helling R. B. The effect of arabinose-specific enzyme synthesis on recombination in the arabinose genes of Escherichia coli. Genetics. 1967 Nov;57(3):665–675. doi: 10.1093/genetics/57.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herskowitz I., Signer E. R. A site essential for expression of all late genes in bacteriophage lambda. J Mol Biol. 1970 Feb 14;47(3):545–556. doi: 10.1016/0022-2836(70)90321-9. [DOI] [PubMed] [Google Scholar]
  8. Naha P. M. Cis-trans position effect of certain genes controlling tail protein in bacteriophage lambda. Virology. 1968 Nov;36(3):519–521. doi: 10.1016/0042-6822(68)90181-5. [DOI] [PubMed] [Google Scholar]
  9. Nijkamp H. J., Bovre K., Szybalski W. Controls of rightward transcription in coliphage lambda. J Mol Biol. 1970 Dec 28;54(3):599–604. doi: 10.1016/0022-2836(70)90130-0. [DOI] [PubMed] [Google Scholar]
  10. Oda K. I., Sakakibara Y., Tomizawa J. I. Regulation of transcription of the lambda bacteriophage genome. Virology. 1969 Dec;39(4):901–918. doi: 10.1016/0042-6822(69)90026-9. [DOI] [PubMed] [Google Scholar]
  11. Parkinson J. S. Genetics of the left arm of the chromosome of bacteriophage lambda. Genetics. 1968 Jul;59(3):311–325. doi: 10.1093/genetics/59.3.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ptashne M., Hopkins N. The operators controlled by the lambda phage repressor. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1282–1287. doi: 10.1073/pnas.60.4.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yarmolinsky M. B., Wiesmeyer H. REGULATION BY COLIPHAGE LAMBDA OF THE EXPRESSION OF THE CAPACITY TO SYNTHESIZE A SEQUENCE OF HOST ENZYMES. Proc Natl Acad Sci U S A. 1960 Dec;46(12):1626–1645. doi: 10.1073/pnas.46.12.1626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zissler J. Integration-negative (int) mutants of phage lambda. Virology. 1967 Jan;31(1):189–189. doi: 10.1016/0042-6822(67)90030-x. [DOI] [PubMed] [Google Scholar]

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