Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1972 Feb;9(2):290–296. doi: 10.1128/jvi.9.2.290-296.1972

Origin of the Vaccinia Virus Hemagglutinin 1

Kenneth E Blackman a,2, H Curt Bubel a
PMCID: PMC356296  PMID: 5062680

Abstract

The relationship between the vaccinia virus hemagglutinin and hemadsorption was examined. Hemagglutinin synthesis was temporally related to the appearance of the hemadsorption reaction. Only chicken erythrocytes, which reacted with hemagglutinin, hemadsorbed to infected cells, and both of these reactions were inhibited by Ca2+. The distribution of the vaccinia hemagglutinin and 5′-adenosine monophosphatase, a plasma membrane marker enzyme, in sucrose gradients was similar. Plasma membrane ghosts derived from infected cells hemadsorbed erythrocytes and yielded hemagglutinin upon sonic disruption. These data suggest that the majority of vaccinia hemagglutinin is derived from the plasma-membrane of the infected cell.

Full text

PDF
290

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUBEL H. C., WOLFF D. A. PROFLAVINE INHIBITION OF VACCINIA VIRUS SYNTHESIS. J Bacteriol. 1965 Apr;89:977–983. doi: 10.1128/jb.89.4.977-983.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blackman K. E., Bubel H. C. Poliovirus-induced Cellular Injury. J Virol. 1969 Sep;4(3):203–208. doi: 10.1128/jvi.4.3.203-208.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boone C. W., Ford L. E., Bond H. E., Stuart D. C., Lorenz D. Isolation of plasma membrane fragments from HeLa cells. J Cell Biol. 1969 May;41(2):378–392. doi: 10.1083/jcb.41.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bubel H. C. Protein leakage from mengovirus-infected cells. Proc Soc Exp Biol Med. 1967 Jul;125(3):783–786. doi: 10.3181/00379727-125-32205. [DOI] [PubMed] [Google Scholar]
  5. Buck C. A., Glick M. C., Warren L. Glycopeptides from the surface of control and virus-transformed cells. Science. 1971 Apr 9;172(3979):169–171. doi: 10.1126/science.172.3979.169. [DOI] [PubMed] [Google Scholar]
  6. DRIESSEN J. H., GREENHAM L. W. Haemadsorption in vaccinia-infected tube tissue cultures. Arch Gesamte Virusforsch. 1959;9(1):45–55. doi: 10.1007/BF01241002. [DOI] [PubMed] [Google Scholar]
  7. Dekking F., van Dillen P. Haemadsorption by vaccinia virus and the Vh factor in chickens. J Gen Virol. 1968 May;2(3):451–453. doi: 10.1099/0022-1317-2-3-451. [DOI] [PubMed] [Google Scholar]
  8. GAUSH C. R., YOUNGNER J. S. Studies on the lipids of virusinfected cells. I. Lipid analysis of a soluble hemagglutinin from chorioallantoic membranes infected with vaccinia virus. Virology. 1963 Apr;19:573–579. doi: 10.1016/0042-6822(63)90053-9. [DOI] [PubMed] [Google Scholar]
  9. Inbar M., Sachs L. Structural difference in sites on the surface membrane of normal and transformed cells. Nature. 1969 Aug 16;223(5207):710–712. doi: 10.1038/223710a0. [DOI] [PubMed] [Google Scholar]
  10. Joklik W. K. The poxviruses. Bacteriol Rev. 1966 Mar;30(1):33–66. doi: 10.1128/br.30.1.33-66.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MARCUS P. I. Dynamics of surface modification in myxovirus-infected cells. Cold Spring Harb Symp Quant Biol. 1962;27:351–365. doi: 10.1101/sqb.1962.027.001.033. [DOI] [PubMed] [Google Scholar]
  12. NEFF B. J., ACKERMANN W. W., PRESTON R. E. STUDIES OF VACCINIA HEMAGGLUTININ OBTAINED FROM VARIOUS VACCINIA INFECTED TISSUES. DENSITY GRADIENT CENTRIFUGATION AND ELECTRON MICROSCOPY. Proc Soc Exp Biol Med. 1965 Mar;118:664–671. doi: 10.3181/00379727-118-29934. [DOI] [PubMed] [Google Scholar]
  13. ODA M. VACCINIA VIRUS-HELA CELL INTERACTION. I. THE EFFECT OF IMMUNE SERUM ON THE PRODUCTION OF INFECTIVE VIRUS, COMPLEMENT-FIXING ANTIGEN, AND HEMAGGLUTININ. Virology. 1963 Aug;20:552–558. doi: 10.1016/0042-6822(63)90279-4. [DOI] [PubMed] [Google Scholar]
  14. SEGAL H. L., BRENNER B. M. 5'-Nucleotidase of rat liver microsomes. J Biol Chem. 1960 Feb;235:471–474. [PubMed] [Google Scholar]
  15. SHELOKOV A., VOGEL J. E., CHI L. Hemadsorption (adsorption-hemagglutination) test for viral agents in tissue culture with special reference to influenza. Proc Soc Exp Biol Med. 1958 Apr;97(4):802–809. doi: 10.3181/00379727-97-23884. [DOI] [PubMed] [Google Scholar]
  16. Ueda Y., Ito M., Tagaya I. A specific surface antigen induced by poxvirus. Virology. 1969 May;38(1):180–182. doi: 10.1016/0042-6822(69)90141-x. [DOI] [PubMed] [Google Scholar]
  17. Weiss L. Studies on cellular adhesion in tissue culture. IX. Electrophoretic mobility and contact phenomena. Exp Cell Res. 1968 Aug-Sep;51(2-3):609–625. doi: 10.1016/0014-4827(68)90149-3. [DOI] [PubMed] [Google Scholar]
  18. YOUNGER J. S., RUBINSTEIN G. Separation and characterization of hemagglutinins from chorioallantoic membranes of embryonated eggs infected with vaccinia virus. Virology. 1962 Mar;16:272–281. doi: 10.1016/0042-6822(62)90248-9. [DOI] [PubMed] [Google Scholar]
  19. ZAJAC I., CROWELL R. L. EFFECT OF ENZYMES ON THE INTERACTION OF ENTEROVIRUSES WITH LIVING HELA CELLS. J Bacteriol. 1965 Mar;89:574–582. doi: 10.1128/jb.89.3.574-582.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. ZWARTOUW H. T., WESTWOOD J. C., APPLEYARD G. Purification of pox viruses by density gradient centrifugation. J Gen Microbiol. 1962 Nov;29:523–529. doi: 10.1099/00221287-29-3-523. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES