Abstract
To determine whether polyamine synthesis is dependent on deoxyribonucleic acid (DNA) synthesis, polyamine levels were estimated after infection of bacterial cells with ultraviolet-irradiated T4 or T4 am N 122, a DNA-negative mutant. Although phage DNA accumulation was restricted to various degrees in comparison to cells infected with T4D, nearly commensurate levels of putrescine and spermidine synthesis were observed after infection, regardless of the rate of phage DNA synthesis. We conclude from these data that polyamine synthesis after infection is independent of phage DNA synthesis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
- COHEN S. S., ARBOGAST R. Chemical studies in host-virus interactions; a comparison of some properties of three mutant pairs of bacterial viruses, T2r and T2r, T4r and T4r, T6r and T6r. J Exp Med. 1950 Jun 1;91(6):619–636. doi: 10.1084/jem.91.6.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen S. S., Hoffner N., Jansen M., Moore M., Raina A. POLYAMINES, RNA SYNTHESIS, AND STREPTOMYCIN LETHALITY IN A RELAXED MUTANT OF E. coli STRAIN 15 TAU. Proc Natl Acad Sci U S A. 1967 Mar;57(3):721–728. doi: 10.1073/pnas.57.3.721. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dion A. S., Cohen S. S. Polyamines in the synthesis of bacteriophage deoxyribonucleic acid. II. Requirement for polyamines in T4 infection of a polyamine auxotroph. J Virol. 1972 Mar;9(3):423–430. doi: 10.1128/jvi.9.3.423-430.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERSHEY A. D. Some minor components of bacteriophage T2 particles. Virology. 1957 Oct;4(2):237–264. doi: 10.1016/0042-6822(57)90061-2. [DOI] [PubMed] [Google Scholar]