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. 1972 Mar;9(3):440–447. doi: 10.1128/jvi.9.3.440-447.1972

Inactivation of Bacteriophage T4 by Ethyl Methanesulfonate: Influence of Host and Viral Genotypes

Usharanjan Ray 1, Lynne Bartenstein 1, John W Drake 1
PMCID: PMC356317  PMID: 4552551

Abstract

Inactivation of bacteriophage T4 by ethyl methanesulfonate (EMS) is a complex process which depends critically upon the conditions of treatment and upon both the viral and the host genotypes. EMS-inactivated particles are capable of multiplicity and cross-reactivation, indicating the need for caution in using EMS in certain types of mutation studies. The pyrimidine dimer excision systems of the phage and the host do not affect the EMS sensitivity of T4, but the T4x+y+ system does. Mutational defects in the deoxyribonucleic acid (DNA) ligase and the DNA polymerase systems both of the virus and of its host also affect viral EMS sensitivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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