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. 1972 Mar;9(3):448–453. doi: 10.1128/jvi.9.3.448-453.1972

Analysis of Simian Virus 40-Induced Transformation of Hamster Kidney Tissue in Vitro VIII. Induction of Infectious Simian Virus 40 from Virogenic Transformed Hamster Cells by Amino Acid Deprivation or Cycloheximide Treatment

Joan C Kaplan 1, Sharon M Wilbert 1, Paul H Black 1
PMCID: PMC356318  PMID: 4335660

Abstract

Clones of virogenic simian virus 40 (SV40)-transformed hamster kidney cells were exposed to medium deficient in the essential amino acids leucine, arginine, or methionine. Infectious virus was induced after deprivation periods of from 24 to 32 hr. The highest yields of infectious SV40 were obtained from cultures deprived for 3 to 4 days. Infectious virus was also induced in cells that were treated with the metabolic inhibitor cycloheximide. Pulse labeling experiments revealed that both protein synthesis and deoxyribonucleic acid (DNA) synthesis were inhibited by concentrations of cycloheximide which were effective for virus induction. It is suggested that inhibition of protein synthesis by either amino acid deprivation or by cycloheximide was responsible for the induction of infectious virus from virogenic cells. We postulate that the inhibition of protein synthesis caused a temporary inhibition of DNA synthesis which resulted in the induction of infectious virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Todaro G. J., Scolnick E. M. Induction of murine C-type viruses from clonal lines of virus-free BALB-3T3 cells. Science. 1971 Oct 8;174(4005):157–159. doi: 10.1126/science.174.4005.157. [DOI] [PubMed] [Google Scholar]
  2. BENNETT L. L., Jr, SMITHERS D., WARD C. T. INHIBITION OF DNA SYNTHESIS IN MAMMALIAN CELLS BY ACTIDIONE. Biochim Biophys Acta. 1964 May 18;87:60–69. doi: 10.1016/0926-6550(64)90047-7. [DOI] [PubMed] [Google Scholar]
  3. BLACK P. H., CRAWFORD E. M., CRAWFORD L. V. THE PURIFICATION OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:381–387. doi: 10.1016/0042-6822(64)90175-8. [DOI] [PubMed] [Google Scholar]
  4. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  5. Ben-Gurion R. Chloramphenicol and puromycin as inducers of colicinogenic bacteria. Biochem Biophys Res Commun. 1970 Sep 30;40(6):1281–1288. doi: 10.1016/0006-291x(70)90005-7. [DOI] [PubMed] [Google Scholar]
  6. Black P. H. An analysis of SV40-induced transformation of hamster kidney tissue in vitro. 3. Persistence of SV40 viral genome in clones of transformed hamster cells. J Natl Cancer Inst. 1966 Oct;37(4):487–493. [PubMed] [Google Scholar]
  7. Bonner J., Dahmus M. E., Fambrough D., Huang R. C., Marushige K., Tuan D. Y. The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action. Science. 1968 Jan 5;159(3810):47–56. doi: 10.1126/science.159.3810.47. [DOI] [PubMed] [Google Scholar]
  8. Burns W. H., Black P. H. Analysis of SV40-induced transformation of hamster kidney tissue in vitro. VI. Characteristics of mitomycin C induction. Virology. 1969 Dec;39(4):625–634. doi: 10.1016/0042-6822(69)90001-4. [DOI] [PubMed] [Google Scholar]
  9. Burns W. H., Black P. H. Analysis of simian virus 40-induced transformation of hamster kidney tissue in vitro. V. Variability of virus recovery from cell clones inducible with mitomycin C and cell fusion. J Virol. 1968 Jun;2(6):606–609. doi: 10.1128/jvi.2.6.606-609.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Culp L. A., Black P. H. DNA synthesis in normal and virus-transformed mammalian cells after methionine deprivation. Biochim Biophys Acta. 1971 Oct 14;247(2):220–232. doi: 10.1016/0005-2787(71)90672-1. [DOI] [PubMed] [Google Scholar]
  11. Ensminger W. D., Tamm I. The step in cellular DNA synthesis blocked by Newcastle disease or mengovirus infection. Virology. 1970 Jan;40(1):152–165. doi: 10.1016/0042-6822(70)90387-9. [DOI] [PubMed] [Google Scholar]
  12. Freed J. J., Schatz S. A. Chromosome aberrations in cultured cells deprived of single essential amino acids. Exp Cell Res. 1969 Jun;55(3):393–409. doi: 10.1016/0014-4827(69)90574-6. [DOI] [PubMed] [Google Scholar]
  13. Goldblum N., Ravid Z., Becker Y. Effect of withdrawal of arginine and other amino acids on the synthesis of tumour and viral antigens of SV 40 virus. J Gen Virol. 1968 Jul;3(1):143–146. doi: 10.1099/0022-1317-3-1-143. [DOI] [PubMed] [Google Scholar]
  14. Hare J. D. Reversible inhibition of DNA synthesis by the arginine analogue canavanine in hamster and mouse cells in vitro. Exp Cell Res. 1969 Nov;58(1):170–174. doi: 10.1016/0014-4827(69)90129-3. [DOI] [PubMed] [Google Scholar]
  15. Henle W., Henle G. Effect of arginine-deficient media on the herpes-type virus associated with cultured Burkitt tumor cells. J Virol. 1968 Mar;2(3):182–191. doi: 10.1128/jvi.2.3.182-191.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kit S., Kurimura T., De Torres R. A., Dubbs D. R. Simian virus 40 deoxyribonucleic acid replication. I. Effect of cycloheximide on the replication of SV40 deoxyribonucleic acid in monkey kidney cells and in heterokaryons of SV40-transformed and susceptible cells. J Virol. 1969 Jan;3(1):25–32. doi: 10.1128/jvi.3.1.25-32.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Littlefield J. W., Jacobs P. S. The relation between DNA and protein synthesis in mouse fibroblasts. Biochim Biophys Acta. 1965 Dec 9;108(4):652–658. doi: 10.1016/0005-2787(65)90061-4. [DOI] [PubMed] [Google Scholar]
  20. Lowy D. R., Rowe W. P., Teich N., Hartley J. W. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science. 1971 Oct 8;174(4005):155–156. doi: 10.1126/science.174.4005.155. [DOI] [PubMed] [Google Scholar]
  21. PIEZ K. A., EAGLE H. The free amino acid pool of cultured human cells. J Biol Chem. 1958 Mar;231(1):533–545. [PubMed] [Google Scholar]
  22. Rothschild H., Black P. H. Analysis of SV40-induced transformation of hamster kidney tissue in vitro. VII. Induction of SV40 virus from transformed hamster cell clones by various agents. Virology. 1970 Sep;42(1):251–256. doi: 10.1016/0042-6822(70)90264-3. [DOI] [PubMed] [Google Scholar]
  23. Rouse H. C., Schlesinger R. W. An arginine-dependent step in the maturation of type 2 adenovirus. Virology. 1967 Nov;33(3):513–522. doi: 10.1016/0042-6822(67)90128-6. [DOI] [PubMed] [Google Scholar]
  24. Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shuster R. C., Weissbach A. Evidence for a new endonuclease synthesized by lambda bacteriophage. J Virol. 1968 Oct;2(10):1096–1101. doi: 10.1128/jvi.2.10.1096-1101.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
  27. Tobey R. A., Ley K. D. Isoleucine-mediated regulation of genome repliction in various mammalian cell lines. Cancer Res. 1971 Jan;31(1):46–51. [PubMed] [Google Scholar]
  28. Watkins J. F. The effects of some metabolic inhibitors on the ability of SV40 virus in transformed cells to be detected by cell fusion. J Cell Sci. 1970 May;6(3):721–737. doi: 10.1242/jcs.6.3.721. [DOI] [PubMed] [Google Scholar]
  29. Winters A. L., Consigli R. A. Effects of arginine deprivation on polyoma virus infection of mouse embryo cultures. J Gen Virol. 1971 Jan;10(1):53–63. doi: 10.1099/0022-1317-10-1-53. [DOI] [PubMed] [Google Scholar]

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