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. 1972 Mar;9(3):479–487. doi: 10.1128/jvi.9.3.479-487.1972

Gene Order of the Poliovirus Capsid Proteins

David Rekosh 1
PMCID: PMC356322  PMID: 4335662

Abstract

Two methods were used to determine the genetic map of the poliovirus capsid proteins. The first method uses pactamycin, a drug which selectively inhibits the initiation of protein synthesis and causes a change in the relative amounts of capsid proteins synthesized. This differential effect on each of the capsid proteins is interpreted as indicating the relative distance of each protein from the initiation site of protein synthesis. The second method involves an analysis of coat precursor molecules released from polyribosomes after a series of short pulses of different length terminated by addition of emetine, a drug which stops all protein synthesis almost immediately after its addition. As the pulse length is increased, each of the capsid proteins within the precursor gains radioactivity with different kinetics. From these kinetics, it is possible to determine the gene order of the capsid proteins within the precursor as well as a rate of protein synthesis. Both methods indicate a gene order for the region of the ribonucleic acid coding for the capsid proteins as (5′ → 3′) VP 4 - VP 2 - VP 3 - VP 1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. Expression of animal virus genomes. Bacteriol Rev. 1971 Sep;35(3):235–241. doi: 10.1128/br.35.3.235-241.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D., Girard M., Darnell J. E. Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology. 1966 Jun;29(2):179–189. doi: 10.1016/0042-6822(66)90024-9. [DOI] [PubMed] [Google Scholar]
  3. Baltimore D., Jacobson M. F., Asso J., Huang A. S. The formation of poliovirus proteins. Cold Spring Harb Symp Quant Biol. 1969;34:741–746. doi: 10.1101/sqb.1969.034.01.083. [DOI] [PubMed] [Google Scholar]
  4. Granboulan N., Girard M. Molecular weight of poliovirus ribonucleic acid. J Virol. 1969 Oct;4(4):475–479. doi: 10.1128/jvi.4.4.475-479.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grollman A. P. Inhibitors of protein biosynthesis. V. Effects of emetine on protein and nucleic acid biosynthesis in HeLa cells. J Biol Chem. 1968 Aug 10;243(15):4089–4094. [PubMed] [Google Scholar]
  6. Grollman A. P. Structural basis for inhibition of protein synthesis by emetine and cycloheximide based on an analogy between ipecac alkaloids and glutarimide antibiotics. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1867–1874. doi: 10.1073/pnas.56.6.1867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huang A. S., Balitmore D. Initiation of polyribosome formation in poliovirus-infected HeLa cells. J Mol Biol. 1970 Feb 14;47(3):275–291. doi: 10.1016/0022-2836(70)90302-5. [DOI] [PubMed] [Google Scholar]
  8. Hunt T., Hunter T., Munro A. Control of haemoglobin synthesis: rate of translation of the messenger RNA for the alpha and beta chains. J Mol Biol. 1969 Jul 14;43(1):123–133. doi: 10.1016/0022-2836(69)90083-7. [DOI] [PubMed] [Google Scholar]
  9. Jacobson M. F., Asso J., Baltimore D. Further evidence on the formation of poliovirus proteins. J Mol Biol. 1970 May 14;49(3):657–669. doi: 10.1016/0022-2836(70)90289-5. [DOI] [PubMed] [Google Scholar]
  10. Jacobson M. F., Baltimore D. Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein. J Mol Biol. 1968 Apr 28;33(2):369–378. doi: 10.1016/0022-2836(68)90195-2. [DOI] [PubMed] [Google Scholar]
  11. Jacobson M. F., Baltimore D. Polypeptide cleavages in the formation of poliovirus proteins. Proc Natl Acad Sci U S A. 1968 Sep;61(1):77–84. doi: 10.1073/pnas.61.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lacroute F., Stent G. S. Peptide chain growth of -galactosidase in Escherichia coli. J Mol Biol. 1968 Jul 14;35(1):165–173. doi: 10.1016/s0022-2836(68)80044-0. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Perlman S., Penman S. Mitochondrial protein synthesis: resistance to emetine and response to RNA synthesis inhibitors. Biochem Biophys Res Commun. 1970 Aug 24;40(4):941–948. doi: 10.1016/0006-291x(70)90994-0. [DOI] [PubMed] [Google Scholar]
  15. Taber R., Rekosh D., Baltimore D. Effect of pactamycin on synthesis of poliovirus proteins: a method for genetic mapping. J Virol. 1971 Oct;8(4):395–401. doi: 10.1128/jvi.8.4.395-401.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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