Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1972 Mar;9(3):510–518. doi: 10.1128/jvi.9.3.510-518.1972

Regulation of λrex Expression After Infection of Escherichia coli K by Lambda Bacteriophage

Lazarus Astrachan 1, Joan F Miller 1
PMCID: PMC356326  PMID: 4552553

Abstract

The ability of Escherichia coli K to support bacteriophage T4rII replication starts to decline at 3 to 6 min after infection by λ. This inhibition appears to depend on expression of the λrex+ gene, since little inhibition was observed following infection by a λrex mutant or by a hybrid bacteriophage λi434 which lacks a functional rex gene. For promotion of the synthesis of rex product, cII+ and N+ genes are required and can act trans, whereas cY+, also required, must be cis to a rex+ gene. These genes presumably play a role in the transcription of the cI-rex operon because they are also known to be required for repressor (cI product) synthesis. Functional cIII, O, P genes are not necessary for ample rex product synthesis. We also observed full rex expression after infection by λsuscI mutants, suggesting that rex and repressor are separate gene products and that repressor is not required for inhibition of T4rII replication. We also report experiments with a rex mutant that is not leaky when in a lysogen but is sufficiently leaky shortly after infection to cause inhibition of T4rII replication.

Full text

PDF
510

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W., DUSSOIX D. Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage lambda. J Mol Biol. 1962 Jul;5:18–36. doi: 10.1016/s0022-2836(62)80058-8. [DOI] [PubMed] [Google Scholar]
  2. BODE V. C., KAISER A. D. REPRESSION OF THE C2 AND C3 CISTRONS OF PHAGE LAMBDA IN A LYSOGENIC BACTERIUM. Virology. 1965 Jan;25:111–121. doi: 10.1016/0042-6822(65)90258-8. [DOI] [PubMed] [Google Scholar]
  3. Benzer S. FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGE. Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):344–354. doi: 10.1073/pnas.41.6.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brachet P., Thomas R. Mapping and functional analysis of y and CII mutants. Mutat Res. 1969 Mar-Apr;7(2):257–260. doi: 10.1016/0027-5107(69)90041-4. [DOI] [PubMed] [Google Scholar]
  5. Buller C. S., Astrachan L. Replication of T4rII bacteriophage in Escherichia coli K-12 (lambda). J Virol. 1968 Apr;2(4):298–307. doi: 10.1128/jvi.2.4.298-307.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CAMPBELL A. Sensitive mutants of bacteriophage lambda. Virology. 1961 May;14:22–32. doi: 10.1016/0042-6822(61)90128-3. [DOI] [PubMed] [Google Scholar]
  7. DUSSOIX D., ARBER W. Host specificity of DNA produced by Escherichia coli. II. Control over acceptance of DNA from infecting phage lambda. J Mol Biol. 1962 Jul;5:37–49. doi: 10.1016/s0022-2836(62)80059-x. [DOI] [PubMed] [Google Scholar]
  8. Dove W. F. Strains of phage lambda in current use. Virology. 1969 Jun;38(2):349–351. doi: 10.1016/0042-6822(69)90378-x. [DOI] [PubMed] [Google Scholar]
  9. Echols H., Green L. Establishment and maintenance of repression by bacteriophage lambda: the role of the cI, cII, and c3 proteins. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2190–2194. doi: 10.1073/pnas.68.9.2190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Eisen H. A., Fuerst C. R., Siminovitch L., Thomas R., Lambert L., Pereira da Silva L., Jacob F. Genetics and physiology of defective lysogeny in K12 (lambda): studies of early mutants. Virology. 1966 Oct;30(2):224–241. doi: 10.1016/0042-6822(66)90098-5. [DOI] [PubMed] [Google Scholar]
  11. Eisen H., Brachet P., Pereira da Silva L., Jacob F. Regulation of repressor expression in lambda. Proc Natl Acad Sci U S A. 1970 Jul;66(3):855–862. doi: 10.1073/pnas.66.3.855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Eisen H., Pereira da Silva L., Jacob F. Sur la régulation précoce du bactériophage lambda. C R Acad Sci Hebd Seances Acad Sci D. 1968 Mar 11;266(11):1176–1178. [PubMed] [Google Scholar]
  13. FRY B. A. Conditions for the infection of Escherichia coli with lambda phage and for the establishment of lysogeny. J Gen Microbiol. 1959 Dec;21:676–684. doi: 10.1099/00221287-21-3-676. [DOI] [PubMed] [Google Scholar]
  14. HERRIOTT R. M., BARLOW J. L. Preparation, purification, and properties of E. coli virus T2. J Gen Physiol. 1952 May;36(1):17–28. doi: 10.1085/jgp.36.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hong J. S., Smith G. R., Ames B. N. Adenosine 3':5'-cyclic monophosphate concentration in the bacterial host regulates the viral decision between lysogeny and lysis. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2258–2262. doi: 10.1073/pnas.68.9.2258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Howard B. D. Phage lambda mutants deficient in r-II exclusion. Science. 1967 Dec 22;158(3808):1588–1589. doi: 10.1126/science.158.3808.1588. [DOI] [PubMed] [Google Scholar]
  17. JACOB F., WOLLMAN E. L. Etude génétique d'un bactériophage tempéré d'Escherichia coli. l. Le système genétique du bactériophage. Ann Inst Pasteur (Paris) 1954 Dec;87(6):653–673. [PubMed] [Google Scholar]
  18. KAISER A. D., JACOB F. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology. 1957 Dec;4(3):509–521. doi: 10.1016/0042-6822(57)90083-1. [DOI] [PubMed] [Google Scholar]
  19. KAISER A. D. Mutations in a temperate bacteriophage affecting its ability to lysogenize Escherichia coli. Virology. 1957 Feb;3(1):42–61. doi: 10.1016/0042-6822(57)90022-3. [DOI] [PubMed] [Google Scholar]
  20. KASATIYA S. S., LIEB M. EFFECT OF HEAT SHOCK ON T4RII MULTIPLICATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Dec;88:1585–1589. doi: 10.1128/jb.88.6.1585-1589.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. KELLENBERGER G., ZICHICHI M. L., WEIGLE J. A mutation affecting the DNA content of bacteriophage lambda and its lysogenizing properties. J Mol Biol. 1961 Aug;3:399–408. doi: 10.1016/s0022-2836(61)80053-3. [DOI] [PubMed] [Google Scholar]
  22. Kayajanian G. Deletion mapping of the c3-c2 region of bacteriophage lambda. Virology. 1970 May;41(1):170–174. doi: 10.1016/0042-6822(70)90065-6. [DOI] [PubMed] [Google Scholar]
  23. LIEB M. The establishment of lysogenicity in Escherichia coli. J Bacteriol. 1953 Jun;65(6):642–651. doi: 10.1128/jb.65.6.642-651.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Oppenheim A. B., Neubauer Z., Calef E. The antirepressor: a new element in the regulation of protein synthesis. Nature. 1970 Apr 4;226(5240):31–32. doi: 10.1038/226031a0. [DOI] [PubMed] [Google Scholar]
  25. Packman S., Sly W. S. Constitutive lambda DNA replication by lambda-C17, a regulatory mutant related to virulence. Virology. 1968 Apr;34(4):778–789. doi: 10.1016/0042-6822(68)90099-8. [DOI] [PubMed] [Google Scholar]
  26. Ptashne M., Hopkins N. The operators controlled by the lambda phage repressor. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1282–1287. doi: 10.1073/pnas.60.4.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ravin V. K., Poluchina G. N. Mutants of phage lambda unable to exclude phage T4rII. Virology. 1968 Aug;35(4):616–617. doi: 10.1016/0042-6822(68)90292-4. [DOI] [PubMed] [Google Scholar]
  28. Reichardt L., Kaiser A. D. Control of lambda repressor synthesis. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2185–2189. doi: 10.1073/pnas.68.9.2185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Signer E. R. Plasmid formation: a new mode of lysogeny by phase lambda. Nature. 1969 Jul 12;223(5202):158–160. doi: 10.1038/223158a0. [DOI] [PubMed] [Google Scholar]
  30. Silva L. H., Jacob F. Etude genétique d'une mutation modifiant la sensibilité à l'immunité chez le bacteriophage lambda. Ann Inst Pasteur (Paris) 1968 Aug;115(2):145–158. [PubMed] [Google Scholar]
  31. Soller A., Epstein H. T. Biochemical and immunological aspects of the exclusion of lambda by superinfection with T4. Virology. 1965 Aug;26(4):715–726. doi: 10.1016/0042-6822(65)90335-1. [DOI] [PubMed] [Google Scholar]
  32. Strack H. B., Kayser M., Holder S. Reduced immunity in lysogens of bacteriophage lambda due to a mutation in the prophage. Virology. 1970 Nov;42(3):707–716. doi: 10.1016/0042-6822(70)90316-8. [DOI] [PubMed] [Google Scholar]
  33. Taylor K., Hradecna Z., Szybalski W. Asymmetric distribution of the transcribing regions on the complementary strands of coliphage lambda DNA. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1618–1625. doi: 10.1073/pnas.57.6.1618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Tomizawa J., Ogawa T. Inhibition of growth of rII mutants of bacteriophage T4 by immunity substance of bacteriophage lambda. J Mol Biol. 1967 Jan 28;23(2):277–280. doi: 10.1016/s0022-2836(67)80033-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES