Abstract
The endonucleolytic action of a deoxyribonuclease activity in rabbitpox and vaccinia virus was established by change in sedimentation rate of denatured 3H-lambda deoxyribonucleic acid substrate. The presence of two deoxyribonuclease activities in pox-virus is confirmed. Exo- and endonuclease activities are unmasked by treatment of purified virus with the detergent Nonidet P-40 and further enhanced by treatment of viral “cores” with trypsin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Birnboim H. The use of gel filtration to distinguish between endonucleolytic and exonucleolytic types of degradation. Biochim Biophys Acta. 1966 Apr 18;119(1):198–200. doi: 10.1016/0005-2787(66)90052-9. [DOI] [PubMed] [Google Scholar]
- Burlingham B. T., Doerfler W., Pettersson U., Philipson L. Adenovirus endonuclease: association with the penton of adenovirus type 2. J Mol Biol. 1971 Aug 28;60(1):45–64. doi: 10.1016/0022-2836(71)90446-3. [DOI] [PubMed] [Google Scholar]
- GEIDUSCHEK E. P., DANIELS A. A SIMPLE ASSAY FOR DNA ENDONUCLEASES. Anal Biochem. 1965 Apr;11:133–137. doi: 10.1016/0003-2697(65)90052-7. [DOI] [PubMed] [Google Scholar]
- Katz E., Moss B. Vaccinia virus structural polypeptide derived from a high-molecular-weight precursor: formation and integration into virus particles. J Virol. 1970 Dec;6(6):717–726. doi: 10.1128/jvi.6.6.717-726.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mizutani S., Boettiger D., Temin H. M. A DNA-depenent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus. Nature. 1970 Oct 31;228(5270):424–427. doi: 10.1038/228424a0. [DOI] [PubMed] [Google Scholar]
- Pogo B. G., Dales S. Two deoxyribonuclease activities within purified vaccinia virus. Proc Natl Acad Sci U S A. 1969 Jul;63(3):820–827. doi: 10.1073/pnas.63.3.820. [DOI] [PMC free article] [PubMed] [Google Scholar]