Abstract
A simple purification procedure for encephalomyocarditis virus is described, yielding material suitable for extraction of viral ribonucleic acid for use as messenger in animal cell-free systems.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boime I., Aviv H., Leder P. Protein synthesis directed by encephalomyocarditis virus RNA. II. The in vitro synthesis of high molecular weight proteins and elements of the viral capsid. Biochem Biophys Res Commun. 1971 Nov 5;45(3):788–795. doi: 10.1016/0006-291x(71)90486-4. [DOI] [PubMed] [Google Scholar]
- Burness A. T., Clothier F. W. Particle weight and other biophysical properties of encephalomyocarditis virus. J Gen Virol. 1970 Mar;6(3):381–393. doi: 10.1099/0022-1317-6-3-381. [DOI] [PubMed] [Google Scholar]
- Burness A. T. Purification of Encephalomyocarditis virus. J Gen Virol. 1969 Sep;5(2):291–303. doi: 10.1099/0022-1317-5-2-291. [DOI] [PubMed] [Google Scholar]
- Dobos P., Kerr I. M., Martin E. M. Synthesis of capsid and noncapsid viral proteins in response to encephalomyocarditis virus ribonucleic acid in animal cell-free systems. J Virol. 1971 Oct;8(4):491–499. doi: 10.1128/jvi.8.4.491-499.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunker A. K., Rueckert R. R. Fragments generated by pH dissociation of ME-virus and their relation to the structure of the virion. J Mol Biol. 1971 May 28;58(1):217–235. doi: 10.1016/0022-2836(71)90242-7. [DOI] [PubMed] [Google Scholar]
- FAULKNER P., MARTIN E. M., SVED S., VALENTINE R. C., WORK T. S. Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 2. The isolation, crystallization and chemical characterization of mouse encephalomyocarditis virus. Biochem J. 1961 Sep;80:597–605. doi: 10.1042/bj0800597. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Granboulan N., Girard M. Molecular weight of poliovirus ribonucleic acid. J Virol. 1969 Oct;4(4):475–479. doi: 10.1128/jvi.4.4.475-479.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kerr I. M., Martin E. M. Virus protein synthesis in animal cell-free systems: nature of the products synthesized in resonse to ribonucleic acid of encephalomyocarditis virus. J Virol. 1971 Apr;7(4):438–447. doi: 10.1128/jvi.7.4.438-447.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Leberman R. The isolation of plant viruses by means of "simple" coacervates. Virology. 1966 Nov;30(3):341–347. doi: 10.1016/0042-6822(66)90112-7. [DOI] [PubMed] [Google Scholar]
- MARTIN E. M., MALEC J., SVED S., WORK T. S. Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 1. Encephalomyocarditis virus in Krebs II mouse-ascites-tumour cells. Biochem J. 1961 Sep;80:585–597. doi: 10.1042/bj0800585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McSharry J., Benzinger R. Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation". Virology. 1970 Mar;40(3):745–746. doi: 10.1016/0042-6822(70)90219-9. [DOI] [PubMed] [Google Scholar]
- Skehel J. J. Estimations of the molecular weight of the influenza virus genome. J Gen Virol. 1971 May;11(2):103–109. doi: 10.1099/0022-1317-11-2-103. [DOI] [PubMed] [Google Scholar]
- Smith A. E., Marcker K. A., Mathews M. B. Translation of RNA from encephalomyocarditis virus in a mammalian cell-free system. Nature. 1970 Jan 10;225(5228):184–187. doi: 10.1038/225184a0. [DOI] [PubMed] [Google Scholar]
- Vande Woude G. F., Bachrach H. L. Number and molecular weights of foot-and-mouth disease virus capsid proteins and the effects of maleylation. J Virol. 1971 Feb;7(2):250–259. doi: 10.1128/jvi.7.2.250-259.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]