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. 1972 May;9(5):804–812. doi: 10.1128/jvi.9.5.804-812.1972

Regulation of Bacteriophage T5 Development by ColI Factors

R W Moyer 1, A S Fu 1, C Szabo 1
PMCID: PMC356377  PMID: 4554465

Abstract

The I-type colicinogenic factor ColIb transforms Escherichia coli from a permissive to a nonpermissive host for bacteriophage T5 reproduction by preventing complete expression of the phage genome. T5-infected ColIb+ cells synthesize only class I (early) phage protein and ribonucleic acid (RNA). Neither phage-specific class II proteins [associated with viral deoxyribonucleic acid (DNA) replication] nor class III proteins (phage structural components) are formed due to the failure of the infected ColIb+ cells to synthesize class II or class III phage-specific messenger RNA. Comparable studies with T5-infected cells colicinogenic for the related ColIa factor revealed no decrease in the yield of progeny phage although the presence of the ColIa factor leads to a significant reduction in the amount of phage-directed class III protein synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boon T. Inactivation of ribosomes in vitro by colicin E 3 . Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421–2425. doi: 10.1073/pnas.68.10.2421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bowman C. M., Sidikaro J., Nomura M. Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells. Nat New Biol. 1971 Dec 1;234(48):133–137. doi: 10.1038/newbio234133a0. [DOI] [PubMed] [Google Scholar]
  3. Fairbanks G., Jr, Levinthal C., Reeder R. H. Analysis of C14-labeled proteins by disc electrophoresis. Biochem Biophys Res Commun. 1965 Aug 16;20(4):393–399. doi: 10.1016/0006-291x(65)90589-9. [DOI] [PubMed] [Google Scholar]
  4. Hendrickson H. E., McCorquodale D. J. Genetic and physiological studies of bacteriophage T5. 2. The relationship between phage DNA synthesis and protein synthesis in T5-infected cells. Biochem Biophys Res Commun. 1971 May 21;43(4):735–740. doi: 10.1016/0006-291x(71)90677-2. [DOI] [PubMed] [Google Scholar]
  5. Jacquemin-Sablon A., Richardson C. C. Analysis of the interruptions in bacteriophage T5 DNA. J Mol Biol. 1970 Feb 14;47(3):477–493. doi: 10.1016/0022-2836(70)90316-5. [DOI] [PubMed] [Google Scholar]
  6. Konisky J., Richards F. M. Characterization of colicin Ia and colicin Ib. Purification and some physical properties. J Biol Chem. 1970 Jun 10;245(11):2972–2978. [PubMed] [Google Scholar]
  7. LANNI Y. T. Invasion by bacteriophage T5. I. Some basic kinetic features. Virology. 1960 Apr;10:501–513. doi: 10.1016/0042-6822(60)90132-x. [DOI] [PubMed] [Google Scholar]
  8. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  9. Lanni Y. T. First-step-transfer deoxyribonucleic acid of bacteriophage T5. Bacteriol Rev. 1968 Sep;32(3):227–242. doi: 10.1128/br.32.3.227-242.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levisohn R., Konisky J., Nomura M. Interaction of colicins with bacterial cells. IV. Immunity breakdown studied with colicins Ia and Ib. J Bacteriol. 1968 Sep;96(3):811–821. doi: 10.1128/jb.96.3.811-821.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MCCORQUODALE D. J., LANNI Y. T. MOLECULAR ASPECTS OF DNA TRANSFER FROM PHAGE T5 TO HOST CELLS. I. CHARACTERIZATION OF FIRST-STEP-TRANSFER MATERIAL. J Mol Biol. 1964 Oct;10:10–18. doi: 10.1016/s0022-2836(64)80023-1. [DOI] [PubMed] [Google Scholar]
  12. McCorquodale D. J., Buchanan J. M. Patterns of protein synthesis in T5-infected Escherichia coli. J Biol Chem. 1968 May 25;243(10):2550–2559. [PubMed] [Google Scholar]
  13. Morrison T. G., Malamy M. H. T7 translational control mechanisms and their inhibiton by F factors. Nat New Biol. 1971 May 12;231(19):37–41. doi: 10.1038/newbio231037a0. [DOI] [PubMed] [Google Scholar]
  14. Moyer R. W., Buchanan J. M. Effect of calcium ions on synthesis of T5-specific proteins. J Biol Chem. 1970 Nov 25;245(22):5897–5903. [PubMed] [Google Scholar]
  15. Moyer R. W., Buchanan J. M. Effect of calcium ions on synthesis of T5-specific ribonucleic acid. J Biol Chem. 1970 Nov 25;245(22):5904–5913. [PubMed] [Google Scholar]
  16. Nisioka T., Ozeki H. Early abortive lysis by phage BF23 in Escherichia coli K-12 carrying the colicin Ib factor. J Virol. 1968 Nov;2(11):1249–1254. doi: 10.1128/jvi.2.11.1249-1254.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nomura M. Colicins and related bacteriocins. Annu Rev Microbiol. 1967;21:257–284. doi: 10.1146/annurev.mi.21.100167.001353. [DOI] [PubMed] [Google Scholar]
  18. Orr C. W., Herriott S. T., Bessman M. J. The enzymology of virus-infected bacteria. VII. A new deoxyribonucleic acid polymerase induced by bacteriophage T5. J Biol Chem. 1965 Dec;240(12):4652–4658. [PubMed] [Google Scholar]
  19. Pispa J. P., Sirbasku D. A., Buchanan J. M. Patterns of ribonucleic acid synthesis in T5-infected Escherichia coli. IV. Examination of the role of deoxyribonucleic acid replication. J Biol Chem. 1971 Mar 25;246(6):1658–1664. [PubMed] [Google Scholar]
  20. Sirbasku D. A., Buchanan J. M. Patterns of ribonucleic acid synthesis in T5-infected Escherichia coli. II. Separation of high molecular weight ribonucleic acid species by disc electrophoresis on acrylamide gel columns. J Biol Chem. 1970 May 25;245(10):2679–2692. [PubMed] [Google Scholar]
  21. Strobel M., Nomura M. Restriction of the growth of bacteriophage BF23 by a colicine I (Col I-P9) factor. Virology. 1966 Apr;28(4):763–765. doi: 10.1016/0042-6822(66)90263-7. [DOI] [PubMed] [Google Scholar]
  22. Summers W. C. A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate. Anal Biochem. 1970 Feb;33(2):459–463. doi: 10.1016/0003-2697(70)90316-7. [DOI] [PubMed] [Google Scholar]
  23. Summers W. C., Jakes K. Phage T7 lysozyme mRNA transcription and translation in vivo and in vitro. Biochem Biophys Res Commun. 1971 Oct 15;45(2):315–320. doi: 10.1016/0006-291x(71)90820-5. [DOI] [PubMed] [Google Scholar]
  24. Summers W. C. The process of infection with coliphage T7. I. Characterization of T7 RNA by polyacrylamide gel electrophoretic analysis. Virology. 1969 Oct;39(2):175–181. doi: 10.1016/0042-6822(69)90037-3. [DOI] [PubMed] [Google Scholar]

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