Abstract
Deoxyribonucleic acid (DNA) extracted from purified virions of Shope fibroma virus (SFV) (by using DNA from Microccocus lysodeikticus as marker) had a buoyant density of 1.6996 ± 0.0003 g/ml), hence a guanine plus cytosine (G + C) content of 40.4 ± 0.3%, which is close to the G + C content of the DNA of susceptible rabbit cells (40.9 ± 0.4%) and different from that of vaccinia virus DNA (35.5 ± 0.4%). For the determination of the molecular weight of DNA, SFV and vaccinia purified virions, treated with Pronase and detergent, were cosedimented in sucrose density gradients. Results showed that SFV-DNA has a molecular weight of about 153 × 106 daltons. By electron microscopy, only one molecule corresponding to this value was observed (its length was 80.3 μm). The others had a median size of 49.8 μm ± 0.9.
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- BURGI E., HERSHEY A. D. Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963 Jul;3:309–321. doi: 10.1016/s0006-3495(63)86823-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freifelder D. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. J Mol Biol. 1970 Dec 28;54(3):567–577. doi: 10.1016/0022-2836(70)90127-0. [DOI] [PubMed] [Google Scholar]
- HAREL J., CONSTANTIN T. Sur la malignité des tumeurs provoquées par le virus fibromateux de Shope chez le lapin nouveauné et le lapin adulte traite par des doses massives de cortisone. Bull Assoc Fr Etud Cancer. 1954;41(4):482–497. [PubMed] [Google Scholar]
- Hearst J. E., Vinograd J. A THREE-COMPONENT THEORY OF SEDIMENTATION EQUILIBRIUM IN A DENSITY GRADIENT. Proc Natl Acad Sci U S A. 1961 Jul;47(7):999–1004. doi: 10.1073/pnas.47.7.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOKLIK W. K. The purification fo four strains of poxvirus. Virology. 1962 Sep;18:9–18. doi: 10.1016/0042-6822(62)90172-1. [DOI] [PubMed] [Google Scholar]
- Jacquemont B., Precosta P., Gautheron D. Isolement et quelques propriétés du DNA du virus du fibrome de Shope. Bull Soc Chim Biol (Paris) 1969 Jun 26;51(2):225–244. [PubMed] [Google Scholar]
- Jacquemont B., Richard M. H., Grange J. Failure to detect homology between the DNA of the Shope fibroma virus and the DNA of the sensitive cell. J Gen Virol. 1971 Mar;10(3):237–242. doi: 10.1099/0022-1317-10-3-237. [DOI] [PubMed] [Google Scholar]
- Jungwirth C., Dawid I. B. Vaccinia DNA: separation of viral from host cell DNA. Arch Gesamte Virusforsch. 1967;20(4):464–468. doi: 10.1007/BF01275228. [DOI] [PubMed] [Google Scholar]
- McCrea J. F., Lipman M. B. Strand-length measurements of normal and 5-iodo-2'-deoxyuridine-treated vaccinia virus deoxyribonucleic acid released by the Kleinschmidt method. J Virol. 1967 Oct;1(5):1037–1044. doi: 10.1128/jvi.1.5.1037-1044.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
- Sarov I., Becker Y. Studies on vaccinia virus DNA. Virology. 1967 Nov;33(3):369–375. doi: 10.1016/0042-6822(67)90112-2. [DOI] [PubMed] [Google Scholar]