Abstract
The envelopes of HVJ (Sendai virus) virions were solubilized with alkali-Emasol treatment. The solubilized envelope subunit(s) associated with hemagglutination-inhibiting antibody blocking, neuraminidase, and low hemagglutinating (HA) activities had a sedimentation coefficient of 8.8S. Envelope fragment-like structures were assembled from the solubilized subunits after Emasol was removed by gel filtration. These reassembled envelope particles with HA activity had cell-fusion activity as well as hemolytic activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the reassembled particles revealed that they mainly consisted of two kinds of polypeptides.
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- ACKERS G. K. MOLECULAR EXCLUSION AND RESTRICTED DIFFUSION PROCESSES IN MOLECULAR-SIEVE CHROMATOGRAPHY. Biochemistry. 1964 May;3:723–730. doi: 10.1021/bi00893a021. [DOI] [PubMed] [Google Scholar]
- Content J., Duesberg P. H. Electrophoretic distribution of the proteins and glycoproteins of influenza virus and Sendai virus. J Virol. 1970 Dec;6(6):707–716. doi: 10.1128/jvi.6.6.707-716.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREEN W. A., KAY C. M. THE INFLUENCE OF BUFFER COMPOSITION ON THE HYDRODYNAMIC BEHAVIOR OF FETUIN. Arch Biochem Biophys. 1963 Sep;102:359–366. doi: 10.1016/0003-9861(63)90242-x. [DOI] [PubMed] [Google Scholar]
- HARRIS H., WATKINS J. F. HYBRID CELLS DERIVED FROM MOUSE AND MAN: ARTIFICIAL HETEROKARYONS OF MAMMALIAN CELLS FROM DIFFERENT SPECIES. Nature. 1965 Feb 13;205:640–646. doi: 10.1038/205640a0. [DOI] [PubMed] [Google Scholar]
- Hosaka Y. Biological activities of sonically treated Sendai virus. J Gen Virol. 1970 Jul;8(1):43–54. doi: 10.1099/0022-1317-8-1-43. [DOI] [PubMed] [Google Scholar]
- Hosaka Y. Isolation and structure of the nucleocapsid of HVJ. Virology. 1968 Jul;35(3):445–457. doi: 10.1016/0042-6822(68)90223-7. [DOI] [PubMed] [Google Scholar]
- Hosaka Y., Kitano H., Ikeguchi S. Studies on the pleomorphism of HVJ virons. Virology. 1966 Jun;29(2):205–221. doi: 10.1016/0042-6822(66)90027-4. [DOI] [PubMed] [Google Scholar]
- Knowles B. B., Jensen F. C., Steplewski Z., Koprowski H. Rescue of infectious SV40 after fusion between dfferent SV 40-transformed cells. Proc Natl Acad Sci U S A. 1968 Sep;61(1):42–45. doi: 10.1073/pnas.61.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laver W. G., Valentine R. C. Morphology of the isolated hemagglutinin and neuraminidase subunits of influenza virus. Virology. 1969 May;38(1):105–119. doi: 10.1016/0042-6822(69)90132-9. [DOI] [PubMed] [Google Scholar]
- MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
- Maizel J. V., Jr Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins. Science. 1966 Feb 25;151(3713):988–990. doi: 10.1126/science.151.3713.988. [DOI] [PubMed] [Google Scholar]
- Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
- Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1970 Nov;6(5):677–684. doi: 10.1128/jvi.6.5.677-684.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Okada Y., Murayama F. Multinucleated giant cell formation by fusion between cells of two different strains. Exp Cell Res. 1965 Oct;40(1):154–158. doi: 10.1016/0014-4827(65)90303-4. [DOI] [PubMed] [Google Scholar]
- Spiro R. G. Studies on fetuin, a glycoprotein of fetal serum. I. Isolation, chemical composition, and physiochemical properties. J Biol Chem. 1960 Oct;235(10):2860–2869. [PubMed] [Google Scholar]
- Tozawa H., Bauer H., Graf T., Gelderblom H. Strain-specific antigen of the avian leukosis sarcoma virus group. I. Isolation and immunological characterization. Virology. 1970 Mar;40(3):530–539. doi: 10.1016/0042-6822(70)90196-0. [DOI] [PubMed] [Google Scholar]
- WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
- Webster R. G., Darlington R. W. Disruption of myxoviruses with Tween 20 and isolation of biologically active hemagglutinin and neuraminidase subunits. J Virol. 1969 Aug;4(2):182–187. doi: 10.1128/jvi.4.2.182-187.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Webster R. G. Estimation of the molecular weights of the polypeptide chains from the isolated hemagglutinin and neuraminidase subunits of influenza viruses. Virology. 1970 Mar;40(3):643–654. doi: 10.1016/0042-6822(70)90209-6. [DOI] [PubMed] [Google Scholar]
- Yerganian G., Nell M. B. Hybridization of dwarf hamster cells by UV-inactivated Sendai virus. Proc Natl Acad Sci U S A. 1966 May;55(5):1066–1073. doi: 10.1073/pnas.55.5.1066. [DOI] [PMC free article] [PubMed] [Google Scholar]