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. 1972 May;9(5):842–850. doi: 10.1128/jvi.9.5.842-850.1972

Solubilization of Envelopes of HVJ (Sendai virus) with Alkali-Emasol Treatment and Reassembly of Envelope Particles with Removal of the Detergent

Kazufumi Shimizu a,1, Yashuhiro Hosaka a, Yohko K Shimizu a
PMCID: PMC356382  PMID: 4337169

Abstract

The envelopes of HVJ (Sendai virus) virions were solubilized with alkali-Emasol treatment. The solubilized envelope subunit(s) associated with hemagglutination-inhibiting antibody blocking, neuraminidase, and low hemagglutinating (HA) activities had a sedimentation coefficient of 8.8S. Envelope fragment-like structures were assembled from the solubilized subunits after Emasol was removed by gel filtration. These reassembled envelope particles with HA activity had cell-fusion activity as well as hemolytic activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the reassembled particles revealed that they mainly consisted of two kinds of polypeptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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