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. 1972 Jun;9(6):981–989. doi: 10.1128/jvi.9.6.981-989.1972

Genetic and Physiological Studies of Bacteriophage T5 III. Patterns of Deoxyribonucleic Acid Synthesis Induced by Mutants of T5 and the Identification of Genes Influencing the Appearance of Phage-Induced Dihydrofolate Reductase and Deoxyribonuclease 1

Herbert E Hendrickson a,2, D James McCorquodale a
PMCID: PMC356404  PMID: 4556511

Abstract

Patterns of deoxyribonucleic acid (DNA) metabolism in nonpermissive cells infected with amber mutants representing 29 genes of T5 are reported. A group of 7 contiguous genes are essential for the synthesis of phage DNA, whereas 20 other genes, when defective, permit varying degrees of phage DNA synthesis. Two further genes are essential for complete transfer of phage DNA to host cells, and therefore indirectly do not permit the synthesis of phage DNA. The structural genes for an early T5 deoxyribonuclease and for T5 DNA polymerase, as well as a gene that affects the synthesis of dihydrofolate reductase, have been identified in the genetic map of T5.

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Selected References

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  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bujard H. Location of single-strand interruptions in the DNA of bacteriophage T5. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1167–1174. doi: 10.1073/pnas.62.4.1167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CRAWFORD L. V. Nucleic acid metabolism in Escherichia coli infected with phage T5. Virology. 1959 Apr;7(4):359–374. doi: 10.1016/0042-6822(59)90065-0. [DOI] [PubMed] [Google Scholar]
  4. De Waard A., Paul A. V., Lehman I. R. The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1241–1248. doi: 10.1073/pnas.54.4.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRY B. A., GROS F. The metabolic activities of Escherichia coli during the establishment of lysogeny. J Gen Microbiol. 1959 Dec;21:685–692. doi: 10.1099/00221287-21-3-685. [DOI] [PubMed] [Google Scholar]
  6. Frenkel G. D., Richardson C. C. The deoxyribonuclease induced after infection of Escherichia coli by bacteriophage T5. I. Characterization of the enzyme as a 5'-exonuclease. J Biol Chem. 1971 Aug 10;246(15):4839–4847. [PubMed] [Google Scholar]
  7. Frenkel G. D., Richardson C. C. The deoxyribonuclease induced after infection of Escherichia coli by bacteriophage T5. II. Role of the enzyme in replication of the pahge deoxyribonucleic acid. J Biol Chem. 1971 Aug 10;246(15):4848–4852. [PubMed] [Google Scholar]
  8. Hendrickson H. E., McCorquodale D. J. Genetic and physiological studies of bacteriophage T5. 2. The relationship between phage DNA synthesis and protein synthesis in T5-infected cells. Biochem Biophys Res Commun. 1971 May 21;43(4):735–740. doi: 10.1016/0006-291x(71)90677-2. [DOI] [PubMed] [Google Scholar]
  9. Hendrickson H. E., McCorquodale D. J. Genetic and physiological studies of bacteriophage t5 I. An expanded genetic map of t5. J Virol. 1971 May;7(5):612–618. doi: 10.1128/jvi.7.5.612-618.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jacquemin-Sablon A., Richardson C. C. Analysis of the interruptions in bacteriophage T5 DNA. J Mol Biol. 1970 Feb 14;47(3):477–493. doi: 10.1016/0022-2836(70)90316-5. [DOI] [PubMed] [Google Scholar]
  11. KISLIUK R. L. Studies on the mechanism of formaldehyde incorporation into serine. J Biol Chem. 1957 Aug;227(2):805–814. [PubMed] [Google Scholar]
  12. LANNI Y. T. Invasion by bacteriophage T5. II. Dissociation of calcium-independent and calcium-dependent processes. Virology. 1960 Apr;10:514–529. doi: 10.1016/0042-6822(60)90133-1. [DOI] [PubMed] [Google Scholar]
  13. LANNI Y. T. Lysis inhibition with a mutant of bacteriophage T5. Virology. 1958 Jun;5(3):481–501. doi: 10.1016/0042-6822(58)90041-2. [DOI] [PubMed] [Google Scholar]
  14. Lanni Y. Functions of two genes in the first-step-transfer DNA of bacteriophage T5. J Mol Biol. 1969 Aug 28;44(1):173–183. doi: 10.1016/0022-2836(69)90412-4. [DOI] [PubMed] [Google Scholar]
  15. MATHEWS C. K., COHEN S. S. Virus-induced acquisition of metabolic function. VI. Dihydrofolate reductase, a new phage-induced enzyme. J Biol Chem. 1963 Feb;238:853–855. [PubMed] [Google Scholar]
  16. MCCORQUODALE D. J., LANNI Y. T. MOLECULAR ASPECTS OF DNA TRANSFER FROM PHAGE T5 TO HOST CELLS. I. CHARACTERIZATION OF FIRST-STEP-TRANSFER MATERIAL. J Mol Biol. 1964 Oct;10:10–18. doi: 10.1016/s0022-2836(64)80023-1. [DOI] [PubMed] [Google Scholar]
  17. Mathews C. K. Evidence that bacteriophage-induced dihydrofolate reductase in a viral gene product. J Biol Chem. 1967 Sep 25;242(18):4083–4086. [PubMed] [Google Scholar]
  18. Mathews C. K. Growth of a dihydrofolate reductaseless mutant of bacteriophage T4. J Virol. 1967 Oct;1(5):963–967. doi: 10.1128/jvi.1.5.963-967.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McCorquodale D. J., Buchanan J. M. Patterns of protein synthesis in T5-infected Escherichia coli. J Biol Chem. 1968 May 25;243(10):2550–2559. [PubMed] [Google Scholar]
  20. Orr C. W., Herriott S. T., Bessman M. J. The enzymology of virus-infected bacteria. VII. A new deoxyribonucleic acid polymerase induced by bacteriophage T5. J Biol Chem. 1965 Dec;240(12):4652–4658. [PubMed] [Google Scholar]
  21. PFEFFERKORN E., AMOS H. Deoxyribonucleic acid breakdown and resynthesis in T5 bacteriophage infection. Virology. 1958 Aug;6(1):299–301. doi: 10.1016/0042-6822(58)90083-7. [DOI] [PubMed] [Google Scholar]
  22. Paul A. V., Lehman I. R. The deoxyribonucleases of Escherichia coli. VII. A deoxyribonuclease induced by infection with phage T-5. J Biol Chem. 1966 Jul 25;241(14):3441–3451. [PubMed] [Google Scholar]
  23. Pispa J. P., Buchanan J. M. Synthesis of bacteriophage T5 specific RNA in vitro. Biochim Biophys Acta. 1971 Sep 30;247(1):181–184. doi: 10.1016/0005-2787(71)90823-9. [DOI] [PubMed] [Google Scholar]
  24. STONE A. B., BURTON K. Studies on the deoxyribonucleases of bacteriophage-infected Escherichia coli. Biochem J. 1962 Dec;85:600–606. doi: 10.1042/bj0850600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Steuart C. D., Anand S. R., Bessman M. J. Studies on the synthesis of deoxyribonucleic acid. I. Further purification and properties of the deoxyribonucleic acid polymerase induced by infection of Escherichia coli with bacteriophage T5. J Biol Chem. 1968 Oct 25;243(20):5308–5318. [PubMed] [Google Scholar]
  26. Studier F. W., Hausmann R. Integration of two sets of T7 mutants. Virology. 1969 Nov;39(3):587–588. doi: 10.1016/0042-6822(69)90106-8. [DOI] [PubMed] [Google Scholar]
  27. Warner H. R., Lewis N. The synthesis of deoxycytidylate deaminase and dihydrofolate reductase and its control in Escherichia coli infected with bacteriophage T4 and T-4 amber mutants. Virology. 1966 May;29(1):172–175. doi: 10.1016/0042-6822(66)90208-x. [DOI] [PubMed] [Google Scholar]

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