Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1972 Jun;9(6):1033–1046. doi: 10.1128/jvi.9.6.1033-1046.1972

Organization of Gene Function in Bacillus subtilis Bacteriophage SP82G 1

D MacDonald Green a,2, David Laman b,3
PMCID: PMC356410  PMID: 4113884

Abstract

A generalized assessment of the functions of 26 genes of SP82G, a bacteriophage of Bacillus subtilis, has been made. The production of phage-specific deoxyribonucleic acid (DNA), DNA-filled phage heads, completed phage particles, phage-specific antigen, and developmental aberrations has been examined in lysates of temperature-sensitive mutants grown under selective conditions. The genes show a tendency to occur on the genome in three groups of related function: genes involved with DNA synthesis, with tail synthesis, and with head synthesis.

Full text

PDF
1033

Images in this article

1034Image
on p.1034
1042Image
on p.1042
1043Image
on p.1043
1044Image
on p.1044

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVISON P. F. THE STRUCTURE OF BACTERIOPHAGE SP8. Virology. 1963 Oct;21:146–151. doi: 10.1016/0042-6822(63)90250-2. [DOI] [PubMed] [Google Scholar]
  2. Edgar R. S., Lielausis I. Some steps in the assembly of bacteriophage T4. J Mol Biol. 1968 Mar 14;32(2):263–276. doi: 10.1016/0022-2836(68)90008-9. [DOI] [PubMed] [Google Scholar]
  3. Edgar R. S., Wood W. B. Morphogenesis of bacteriophage T4 in extracts of mutant-infected cells. Proc Natl Acad Sci U S A. 1966 Mar;55(3):498–505. doi: 10.1073/pnas.55.3.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GREEN D. M. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82. J Mol Biol. 1964 Dec;10:438–451. doi: 10.1016/s0022-2836(64)80065-6. [DOI] [PubMed] [Google Scholar]
  5. Gage L. P., Geiduschek E. P. RNA synthesis during bacteriophage SPO1 development: six classes of SPO1 RNA. J Mol Biol. 1971 Apr 28;57(2):279–297. doi: 10.1016/0022-2836(71)90346-9. [DOI] [PubMed] [Google Scholar]
  6. KRIEG D. R. Ethyl methanesulfonate-induced reversion of bacteriophage T4rII mutants. Genetics. 1963 Apr;48:561–580. doi: 10.1093/genetics/48.4.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kahan E. A genetic study of temperature-sensitive mutants of the subtilis phage SP82. Virology. 1966 Dec;30(4):650–660. doi: 10.1016/0042-6822(66)90170-x. [DOI] [PubMed] [Google Scholar]
  8. Kahan E. Early and late gene function in bacteriophage SP82. Virology. 1971 Dec;46(3):634–637. doi: 10.1016/0042-6822(71)90066-3. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K., Mölbert E., Showe M., Kellenberger E. Form-determining function of the genes required for the assembly of the head of bacteriophage T4. J Mol Biol. 1970 Apr 14;49(1):99–113. doi: 10.1016/0022-2836(70)90379-7. [DOI] [PubMed] [Google Scholar]
  10. McAllister W. T. Bacteriophage infection: which end of the SP82G genome goes in first? J Virol. 1970 Feb;5(2):194–198. doi: 10.1128/jvi.5.2.194-198.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meselson M., Stahl F. W., Vinograd J. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581–588. doi: 10.1073/pnas.43.7.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mount D. W., Harris A. W., Fuerst C. R., Siminovitch L. Mutations in bacteriophage lambda affecting particle morphogenesis. Virology. 1968 May;35(1):134–149. doi: 10.1016/0042-6822(68)90313-9. [DOI] [PubMed] [Google Scholar]
  13. Parkinson J. S. Genetics of the left arm of the chromosome of bacteriophage lambda. Genetics. 1968 Jul;59(3):311–325. doi: 10.1093/genetics/59.3.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Studier F. W., Maizel J. V., Jr T7-directed protein synthesis. Virology. 1969 Nov;39(3):575–586. doi: 10.1016/0042-6822(69)90105-6. [DOI] [PubMed] [Google Scholar]
  15. Truffaut N., Revet B., Soulie M. O. Etude comparative des DNA de phages 2C, SP8*, SP82, phi e, SP01 et SP50. Eur J Biochem. 1970 Aug;15(2):391–400. doi: 10.1111/j.1432-1033.1970.tb01020.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES