Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1972 Jul;10(1):9–16. doi: 10.1128/jvi.10.1.9-16.1972

Adenovirus-Associated Virus Multiplication VIII. Analysis of In Vivo Transcription Induced by Complete or Partial Helper Viruses

Barrie J Carter 1, James A Rose 1
PMCID: PMC356419  PMID: 4339201

Abstract

We have analyzed ribonucleic acid (RNA) synthesized in KB cells co-infected with adenovirus-associated virus (AAV) type 2 and either adenovirus type 2 (Ad2) or herpes simplex virus type 1 (HSV-1). With either type of helper virus, synthesis of AAV RNA was readily detected by deoxyribonucleic acid (DNA)-RNA hybridization. As is the case for AAV RNA synthesized with helper Ad2, the AAV RNA synthesized with HSV-1 as helper annealed only to the thymidine-rich (minus) AAV DNA strand. In addition, AAV RNA synthesized with either type of helper (i) contained similar nucleotide sequences as determined by hybridization inhibition tests and (ii) had a mean molecular weight of approximately 7.5 × 105 based on sedimentation in dimethylsulfoxide-sucrose gradients. These experiments suggest that the restricted helper function of HSV-1 is not due to abnormal transcription of the AAV genome. Since the mean molecular weight of AAV RNA is equivalent to 40 to 50% of the AAV genome, as few as two or three AAV RNA species may be transcribed in vivo. In contrast to adenovirus RNA, cleavage of AAV RNA after transcription was not observed.

Full text

PDF
9

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATCHISON R. W., CASTO B. C., HAMMON W. M. ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES. Science. 1965 Aug 13;149(3685):754–756. doi: 10.1126/science.149.3685.754. [DOI] [PubMed] [Google Scholar]
  2. Atchison R. W. The role of herpesviruses in adenovirus-associated virus replication in vitro. Virology. 1970 Sep;42(1):155–162. doi: 10.1016/0042-6822(70)90248-5. [DOI] [PubMed] [Google Scholar]
  3. Berns K. I., Rose J. A. Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands. J Virol. 1970 Jun;5(6):693–699. doi: 10.1128/jvi.5.6.693-699.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blacklow N. R., Hoggan M. D., McClanahan M. S. Adenovirus-associated viruses: enhancement by human herpesviruses. Proc Soc Exp Biol Med. 1970 Sep;134(4):952–954. doi: 10.3181/00379727-134-34919. [DOI] [PubMed] [Google Scholar]
  5. Boucher D. W., Melnick J. L., Mayor H. D. Nonencapsidated infectious DNA of adeno-satellite virus in cells coinfected with herpesvirus. Science. 1971 Sep 24;173(4003):1243–1245. doi: 10.1126/science.173.4003.1243. [DOI] [PubMed] [Google Scholar]
  6. Flanagan J. F. Virus-specific ribonucleic acid synthesis in KB cells infected with herpes simplex virus. J Virol. 1967 Jun;1(3):583–590. doi: 10.1128/jvi.1.3.583-590.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoggan M. D., Blacklow N. R., Rowe W. P. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1467–1474. doi: 10.1073/pnas.55.6.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Parks W. P., Green M., Piña M., Melnick J. L. Physicochemical characterization of adeno-associated satellite virus type 4 and its nucleic acid. J Virol. 1967 Oct;1(5):980–987. doi: 10.1128/jvi.1.5.980-987.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Parsons J. T., Gardner J., Green M. Biochemical studies on adenovirus multiplication, XIX. Resolution of late viral RNA species in the nucleus and cytoplasm. Proc Natl Acad Sci U S A. 1971 Mar;68(3):557–560. doi: 10.1073/pnas.68.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Parsons J. T., Green M. Biochemical studies on adenovirus multiplication. 18. Resolution of early virus-specific RNA species in Ad 2 infected and transformed cells. Virology. 1971 Jul;45(1):154–162. doi: 10.1016/0042-6822(71)90122-x. [DOI] [PubMed] [Google Scholar]
  11. Raskas H. J., Thomas D. C., Green M. Biochemical studies on adenovirus multiplication. XVII. Ribosome synthesis in uninfected and infected KB cells. Virology. 1970 Apr;40(4):893–902. doi: 10.1016/0042-6822(70)90135-2. [DOI] [PubMed] [Google Scholar]
  12. Reich P. R., Forget B. G., Weissman S. M. RNA of low molecular weight in KB cells infected with adenovirus type 2. J Mol Biol. 1966 Jun;17(2):428–439. doi: 10.1016/s0022-2836(66)80153-5. [DOI] [PubMed] [Google Scholar]
  13. Rose J. A., Berns K. I., Hoggan M. D., Koczot F. J. Evidence for a single-stranded adenovirus-associated virus genome: formation of a DNA density hybrid on release of viral DNA. Proc Natl Acad Sci U S A. 1969 Nov;64(3):863–869. doi: 10.1073/pnas.64.3.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rose J. A., Hoggan M. D., Shatkin A. J. Nucleic acid from an adeno-associated virus: chemical and physical studies. Proc Natl Acad Sci U S A. 1966 Jul;56(1):86–92. doi: 10.1073/pnas.56.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rose J. A., Koczot F. Adenovirus-associated virus multiplication. VI. Base compostion of the deoxyribonucleic acid strand species and strand-specific in vivo transcription. J Virol. 1971 Nov;8(5):771–777. doi: 10.1128/jvi.8.5.771-777.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rose J. A., Koczot F. Adenovirus-associated virus multiplication. VII. Helper requirement for viral deoxyribonucleic acid and ribonucleic acid synthesis. J Virol. 1972 Jul;10(1):1–8. doi: 10.1128/jvi.10.1.1-8.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rose J. A., Maizel J. V., Jr, Inman J. K., Shatkin A. J. Structural proteins of adenovirus-associated viruses. J Virol. 1971 Nov;8(5):766–770. doi: 10.1128/jvi.8.5.766-770.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  19. Strauss J. H., Jr, Kelly R. B., Sinsheimer R. L. Denaturation of RNA with dimethyl sulfoxide. Biopolymers. 1968 Jun;6(6):793–807. doi: 10.1002/bip.1968.360060604. [DOI] [PubMed] [Google Scholar]
  20. Wagner E. K., Roizman B. Ribonucleic acid synthesis in cells infected with herpes simplex virus. I. Patterns of ribonucleic acid synthesis in productively infected cells. J Virol. 1969 Jul;4(1):36–46. doi: 10.1128/jvi.4.1.36-46.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES