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. 1972 Jul;10(1):134–141. doi: 10.1128/jvi.10.1.134-141.1972

Characterization of Bacteriophage S13 suN15 Single-Strand and Replicative-Form Deoxyribonucleic Acid

J H Spencer a, R ˘Cerný a,1, E ˘Cerná a,1, A D Delaney a
PMCID: PMC356434  PMID: 4557206

Abstract

The deoxyribonucleic acid (DNA) of bacteriophage S13 was shown to be single-stranded by the criteria of reactivity with formaldehyde, dependence of optical density on ionic strength, broad temperature-absorbance profile, and lack of molar equivalence of the purine and pyrimidine bases. The DNA has a molecular weight of 1.8 × 106 daltons, an S°20 of 24.6 in SSC (0.15 m NaCl plus 0.015 m sodium citrate), and a buoyant density of 1.726 g/cc in CsCl. Electron microscopy showed the molecule to be circular. S13 replicative-form DNA was shown to be a double-stranded, circular molecule with a molecular weight of 3.5 × 106 daltons, an S[ill] of 20.7 in SSC, and a buoyant density in CsCl of 1.710 g/cc. The finding that S13 DNA is slightly more pyrimidine-rich than φX174 DNA but is indistinguishable by all other parameters supports the close genetic relationship between the two bacteriophages.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker R., Tessman I. The circular genetic map of phage S13. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1438–1445. doi: 10.1073/pnas.58.4.1438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benbow R. M., Hutchison C. A., Fabricant J. D., Sinsheimer R. L. Genetic Map of Bacteriophage phiX174. J Virol. 1971 May;7(5):549–558. doi: 10.1128/jvi.7.5.549-558.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHANDLER B., HAYASHI M., HAYASHI M. N., SPIEGELMAN S. CIRCULARITY OF THE REPLICATING FORM OF A SINGLE-STRANDED DNA VIRUS. Science. 1964 Jan 3;143(3601):47–49. doi: 10.1126/science.143.3601.47. [DOI] [PubMed] [Google Scholar]
  4. Cerný R., Cerná E., Spencer J. H. Nucleotide clusters in deoxyribonucleic acids. IV. Pyrimidine oligonucleotides of bacteriophage S13 suN15 DNA and replicative form DNA. J Mol Biol. 1969 Nov 28;46(1):145–156. doi: 10.1016/0022-2836(69)90062-x. [DOI] [PubMed] [Google Scholar]
  5. Darby G., Dumas L. B., Sinsheimer R. L. The structure of the DNA of bacteriophage phiX174. VI. Pyrimidine sequences in the complementary strand of the replicative form. J Mol Biol. 1970 Sep 14;52(2):227–238. doi: 10.1016/0022-2836(70)90027-6. [DOI] [PubMed] [Google Scholar]
  6. FREIFELDER D., KLEINSCHMIDT A. K., SINSHEIMER R. L. ELECTRON MICROSCOPY OF SINGLE-STRANDED DNA: CIRCULARITY OF DNA OF BACTERIOPHAGE PHI-X174. Science. 1964 Oct 9;146(3641):254–255. doi: 10.1126/science.146.3641.254. [DOI] [PubMed] [Google Scholar]
  7. Freifelder D. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. J Mol Biol. 1970 Dec 28;54(3):567–577. doi: 10.1016/0022-2836(70)90127-0. [DOI] [PubMed] [Google Scholar]
  8. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  9. HOFFMANN-BERLING H., MARVIN D. A., DUERWALD H. EIN FAEDIGER DNS-PHAGE (FD) UND EIN SPHAERISCHER RNS-PHAGE (FR), WIRTSSPEZIFISCH FUER MAENNLICHE STAEMME VON E. COLI. 1. PRAEPARATION UND CHEMISCHE EIGENSCHAFTEN VON FD UND FR. Z Naturforsch B. 1963 Nov;18:876–883. [PubMed] [Google Scholar]
  10. Jeng Y., Gelfand D., Hayashi M., Shleser R., Tessman E. S. The eight genes of bacteriophages phi X174 and S13 and comparison of the phage-specified proteins. J Mol Biol. 1970 Apr 28;49(2):521–526. doi: 10.1016/0022-2836(70)90262-7. [DOI] [PubMed] [Google Scholar]
  11. KAY D. The nucleic acid composition of bacteriophage phi R. J Gen Microbiol. 1962 Feb;27:201–207. doi: 10.1099/00221287-27-2-201. [DOI] [PubMed] [Google Scholar]
  12. KLEINSCHMIDT A. K., BURTON A., SINSHEIMER R. L. ELECTRON MICROSCOPY OF THE REPLICATIVE FORM OF THE DNA OF THE BACTERIOPHAGE PHI-X174. Science. 1963 Nov 15;142(3594):961–961. doi: 10.1126/science.142.3594.961. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. MACHATTIE L. A., THOMAS C. A., Jr DNA FROM BACTERIOPHAGE LAMBDA: MOLECULAR LENGTH AND CONFORMATION. Science. 1964 May 29;144(3622):1142–1144. doi: 10.1126/science.144.3622.1142. [DOI] [PubMed] [Google Scholar]
  15. MACLEAN E. C., HALL C. E. Studies on bacteriophage phi-X174 and its DNA by electron microscopy. J Mol Biol. 1962 Mar;4:173–178. doi: 10.1016/s0022-2836(62)80049-7. [DOI] [PubMed] [Google Scholar]
  16. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  17. Poljak R. J., Suruda A. J. The cost proteins of phi-X-174 and S-13. Virology. 1969 Sep;39(1):145–147. doi: 10.1016/0042-6822(69)90358-4. [DOI] [PubMed] [Google Scholar]
  18. Riva S., Barrai I., Cavalli-Sforza L., Falaschi A. Dependence of the buoyant density of single-stranded DNA on base composition. J Mol Biol. 1969 Oct 28;45(2):367–374. doi: 10.1016/0022-2836(69)90111-9. [DOI] [PubMed] [Google Scholar]
  19. SALIVAR W. O., TZAGOLOFF H., PRATT D. SOME PHYSICAL-CHEMICAL AND BIOLOGICAL PROPERTIES OF THE ROD-SHAPED COLIPHAGE M13. Virology. 1964 Nov;24:359–371. doi: 10.1016/0042-6822(64)90173-4. [DOI] [PubMed] [Google Scholar]
  20. Shleser R., Ishiwa H., Mannes B., Tessman E. S. Early replicative form DNA of bacteriophage S13. I. Sucrose gradient analysis of replicative form made by gene IV mutants. J Mol Biol. 1968 May 28;34(1):121–129. doi: 10.1016/0022-2836(68)90238-6. [DOI] [PubMed] [Google Scholar]
  21. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  22. TESSMAN E. S. COMPLEMENTATION GROUPS IN PHAGE S13. Virology. 1965 Feb;25:303–321. doi: 10.1016/0042-6822(65)90208-4. [DOI] [PubMed] [Google Scholar]
  23. TESSMAN E. S., SHLESER R. Genetic recombination between phages S13 and phi-X174. Virology. 1963 Feb;19:239–240. doi: 10.1016/0042-6822(63)90015-1. [DOI] [PubMed] [Google Scholar]
  24. TESSMAN I. Some unusual properties of the nucleic acid in bacteriophages S13 and phi X174. Virology. 1959 Mar;7(3):263–275. doi: 10.1016/0042-6822(59)90197-7. [DOI] [PubMed] [Google Scholar]
  25. Tessman E. S. Mutants of bacteriophage S13 blocked in infectious DNA synthesis. J Mol Biol. 1966 May;17(1):218–236. doi: 10.1016/s0022-2836(66)80104-3. [DOI] [PubMed] [Google Scholar]
  26. Westmoreland B. C., Szybalski W., Ris H. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy. Science. 1969 Mar 21;163(3873):1343–1348. doi: 10.1126/science.163.3873.1343. [DOI] [PubMed] [Google Scholar]
  27. ZAHLER S. A. Some biological properties of bacteriophages S13 and theta X-174. J Bacteriol. 1958 Mar;75(3):310–315. doi: 10.1128/jb.75.3.310-315.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]

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