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. 1972 Aug;10(2):193–201. doi: 10.1128/jvi.10.2.193-201.1972

Isolation and Characterization of Simian Virus 40 Ribonucleic Acid

R A Weinberg a,1, S O Warnaar a,2, E Winocour a
PMCID: PMC356450  PMID: 4342237

Abstract

Deoxyribonucleic acid-ribonucleic acid (RNA) hybridization in formamide was used to isolate simian virus 40-specific RNA. Early in the lytic cycle, a 19S viral RNA species was observed. Late in the lytic cycle, 16S and 19S viral species were found. The 16S and 19S species of viral RNA were localized in the cytoplasm. High-molecular-weight heterogeneous RNA, containing viral sequences, was isolated from the nuclear fraction of infected cells late in the lytic cycle. This RNA may contain non-viral sequences linked to viral sequences. The formamide hybridization technique can be used to isolate intact late lytic viral RNA which is at least 99% pure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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