Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1972 Aug;10(2):211–219. doi: 10.1128/jvi.10.2.211-219.1972

Studies of the Mechanism of Enhancement of Human Adenovirus Infection in Monkey Cells by Simian Virus 40

Stephen G Baum 1, Marshall S Horwitz 1, Jacob V Maizel Jr 1
PMCID: PMC356452  PMID: 4342239

Abstract

The defect which prevents human adenovirus replication in monkey cells and the mechanism whereby this restriction is overcome by coinfection with simian virus 40 (SV40) have been studied. Adenovirus capsid proteins are not synthesized efficiently in monkey cells in the absence of SV40. Adenovirus “enhancement” by SV40 was found to be the product of increased efficiency of replication by a small percentage of cells. This enhancement effect apparently occurred only when SV40 and adenovirus infected the same cells. These findings suggest that the replicative block occurs prior to virion assembly, and an accompanying report seeks to locate the site of restriction more precisely at the post-transcriptional level.

Full text

PDF
211

Images in this article

214Image
on p.214
214Image
on p.214
216Image
on p.216

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACK P. H., ROWE W. P. AN ANALYSIS OF SV40-INDUCED TRANSFORMATION OF HAMSTER KIDNEY TISSUE IN VITRO. I. GENERAL CHARACTERISTICS. Proc Natl Acad Sci U S A. 1963 Oct;50:606–613. doi: 10.1073/pnas.50.4.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baum S. G., Wiese W. H., Reich P. R. Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: formation of adenovirus-specific RNA. Virology. 1968 Feb;34(2):373–376. doi: 10.1016/0042-6822(68)90253-5. [DOI] [PubMed] [Google Scholar]
  3. Fox R. I., Baum S. G. Synthesis of viral ribonucleic acid during restricted adenovirus infection. J Virol. 1972 Aug;10(2):220–227. doi: 10.1128/jvi.10.2.220-227.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Friedman M. P., Lyons M. J., Ginsberg H. S. Biochemical consequences of type 2 adenovirus and Simian virus 40 double infections of African green monkey kidney cells. J Virol. 1970 May;5(5):586–597. doi: 10.1128/jvi.5.5.586-597.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Horwitz M. S., Scharff M. D., Maizel J. V., Jr Synthesis and assembly of adenovirus 2. I. Polypeptide synthesis, assembly of capsomeres, and morphogenesis of the virion. Virology. 1969 Dec;39(4):682–694. doi: 10.1016/0042-6822(69)90006-3. [DOI] [PubMed] [Google Scholar]
  6. Jerkofsky M. A., Rapp F. Cell-mediated complementation of human adenoviruses by simian papovavirus. Proc Soc Exp Biol Med. 1969 Dec;132(3):987–992. doi: 10.3181/00379727-132-34351. [DOI] [PubMed] [Google Scholar]
  7. LEIF R. C., VINOGRAD J. THE DISTRIBUTION OF BUOYANT DENSITY OF HUMAN ERYTHROCYTES IN BOVINE ALBUMIN SOLUTIONS. Proc Natl Acad Sci U S A. 1964 Mar;51:520–528. doi: 10.1073/pnas.51.3.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
  10. McAllister R. M., Goodheart C. R., Mirabal V. Q., Huebner R. J. Human adenoviruses: tumor production in hamsters by type 12 and 18 grown from single plaques. Proc Soc Exp Biol Med. 1966 Jun;122(2):455–458. doi: 10.3181/00379727-122-31160. [DOI] [PubMed] [Google Scholar]
  11. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RABSON A. S., O'CONOR G. T., BEREZESKY I. K., PAUL F. J. ENHANCEMENT OF ADENOVIRUS GROWTH IN AFRICAN GREEN MONKEY KIDNEY CELL CULTURES BY SV40. Proc Soc Exp Biol Med. 1964 May;116:187–190. doi: 10.3181/00379727-116-29197. [DOI] [PubMed] [Google Scholar]
  14. ROWE W. P., BAUM S. G. EVIDENCE FOR A POSSIBLE GENETIC HYBRID BETWEEN ADENOVIRUS TYPE 7 AND SV40 VIRUSES. Proc Natl Acad Sci U S A. 1964 Dec;52:1340–1347. doi: 10.1073/pnas.52.6.1340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rapp F., Feldman L. A., Mandel M. Synthesis of virus deoxyribonucleic acid during abortive infection of simian cells by human adenoviruses. J Bacteriol. 1966 Oct;92(4):931–936. doi: 10.1128/jb.92.4.931-936.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reich P. R., Baum S. G., Rose J. A., Rowe W. P., Weissman S. M. Nucleic acid homology studies of adenovirus type 7-SV40 interactions. Proc Natl Acad Sci U S A. 1966 Feb;55(2):336–341. doi: 10.1073/pnas.55.2.336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reid M. S., Bieleski R. L. A simple apparatus for vertical flat-sheet polyacrylamide gel electrophoresis. Anal Biochem. 1968 Mar;22(3):374–381. doi: 10.1016/0003-2697(68)90278-9. [DOI] [PubMed] [Google Scholar]
  18. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  19. Rowe W. P., Bau S. G. Studies of adenovirus SV40 hybrid viruses. II. Defectiveness of the hybrid particles. J Exp Med. 1965 Nov 1;122(5):955–966. doi: 10.1084/jem.122.5.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rowe W. P., Baum S. G., Pugh E., Hoggan M. D. Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization. J Exp Med. 1965 Nov 1;122(5):943–954. doi: 10.1084/jem.122.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Russell W. C., Skehel J. J. The polypeptides of adenovirus-infected cells. J Gen Virol. 1972 Apr;15(1):45–57. doi: 10.1099/0022-1317-15-1-45. [DOI] [PubMed] [Google Scholar]
  22. White D. O., Scharff M. D., Maizel J. V., Jr The polypeptides of adenovirus. 3. Synthesis in infected cells. Virology. 1969 Jul;38(3):395–406. doi: 10.1016/0042-6822(69)90152-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES