Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1972 Aug;10(2):310–313. doi: 10.1128/jvi.10.2.310-313.1972

Defective Deoxyribonucleic Acid Replication of T4rII Bacteriophage in Lambda-Lysogenic Host Cells 1

Raymonde Szargel a, Channa Shalitin a
PMCID: PMC356463  PMID: 4561406

Abstract

Sedimentation of the replicative deoxyribonucleic acid through alkaline sucrose gradients showed that rII single chains reached the half-mature size at a time when wild-type molecules formed long chains (dimers and trimers of genome size). Long rII single chains could be observed on substitution of tris(hydroxymethyl)aminomethane buffer for Na+K+ phosphate in the growth medium.

Full text

PDF
310

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W., LATASTE-DOROLLE C. [Enlargement of the host area of bacteriophage lambda for Escherichia coli B]. Pathol Microbiol (Basel) 1961;24:1012–1018. [PubMed] [Google Scholar]
  2. Berger H., Kozinski A. W. Suppression of T4D ligase mutations by rIIa and rIIb mutations. Proc Natl Acad Sci U S A. 1969 Nov;64(3):897–904. doi: 10.1073/pnas.64.3.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlson K., Kozinski A. W. Parent-to-progeny transfer and recombination of T4rII bacteriophage. J Virol. 1970 Sep;6(3):344–352. doi: 10.1128/jvi.6.3.344-352.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frankel F. R. Evidence for long DNA strands in the replicating pool after T4 infection. Proc Natl Acad Sci U S A. 1968 Jan;59(1):131–138. doi: 10.1073/pnas.59.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Frankel F. R. Studies on the nature of replicating DNA in T4-infected Escherichia coli. J Mol Biol. 1966 Jun;18(1):127–143. doi: 10.1016/s0022-2836(66)80081-5. [DOI] [PubMed] [Google Scholar]
  6. GAREN A. Physiological effects of rII mutations in bacteriophage T4. Virology. 1961 Jun;14:151–163. doi: 10.1016/0042-6822(61)90190-8. [DOI] [PubMed] [Google Scholar]
  7. Hosoda J., Mathews E. DNA replication in vivo by polynucleotide-ligase defective mutants of T4. II. Effect of chloramphenicol and mutations in other genes. J Mol Biol. 1971 Jan 28;55(2):155–179. doi: 10.1016/0022-2836(71)90189-6. [DOI] [PubMed] [Google Scholar]
  8. Howard B. D. Phage lambda mutants deficient in r-II exclusion. Science. 1967 Dec 22;158(3808):1588–1589. doi: 10.1126/science.158.3808.1588. [DOI] [PubMed] [Google Scholar]
  9. Iwatsuki N., Okazaki R. Mechanism of DNA chain growth. V. Effect of chloramphenicol on the formation of T4 nascent short DNA chains. J Mol Biol. 1970 Aug 28;52(1):37–44. doi: 10.1016/0022-2836(70)90175-0. [DOI] [PubMed] [Google Scholar]
  10. Karam J. D., Barker B. Properties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene. J Virol. 1971 Feb;7(2):260–266. doi: 10.1128/jvi.7.2.260-266.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Karam J. D. DNA replication of phage T4 rII mutants without polynucleotide ligase (gene 30). Biochem Biophys Res Commun. 1969 Oct 22;37(3):416–422. doi: 10.1016/0006-291x(69)90931-0. [DOI] [PubMed] [Google Scholar]
  12. Kozinski A. W. Molecular recombination in the ligase negative T4 amber mutant. Cold Spring Harb Symp Quant Biol. 1968;33:375–391. doi: 10.1101/sqb.1968.033.01.044. [DOI] [PubMed] [Google Scholar]
  13. Krisch H. M., Shah D. B., Berger H. Replication and recombination in ligase-deficient rII bacteriophage T4D. J Virol. 1971 Apr;7(4):491–498. doi: 10.1128/jvi.7.4.491-498.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller R. C., Kozinski A. W., Litwin S. Molecular Recombination in T4 Bacteriophage Deoxyribonucleic Acid: III. Formation of Long Single Strands During Recombination. J Virol. 1970 Mar;5(3):368–380. doi: 10.1128/jvi.5.3.368-380.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rutberg B., Rutberg L. Bacteriophage-induced functions in Escherichia coli K (lambda) infected with rII mutants of bacteriophage T4. J Bacteriol. 1966 Jan;91(1):76–80. doi: 10.1128/jb.91.1.76-80.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sekiguchi M. Studies on the physiological defect in rII mutants of bacteriophage T4. J Mol Biol. 1966 Apr;16(2):503–522. doi: 10.1016/s0022-2836(66)80188-2. [DOI] [PubMed] [Google Scholar]
  17. Shalitin C., Naot Y. Role of gene 46 in bacteriophage T4 deoxyribonucleic acid synthesis. J Virol. 1971 Aug;8(2):142–153. doi: 10.1128/jvi.8.2.142-153.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Werner R. Mechanism of DNA replication. Nature. 1971 Apr 30;230(5296):570–572. doi: 10.1038/230570a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES