Figure 5. Adsorption of a 0.01 mg/mL solution of mAb-1 to a glass surface pre-coated with polysorbate in PBS buffer pH 7.4, investigated using TIRF, as follows: Tween 20, 0.05 mM (red line) and 1 mM (gray line); Tween 80, 5 µM (blue line) and 1 mM (green line). Reference was to the adsorption of a 0.01 mg/mL solution of mAb-1 to a glass surface (black line).