Figure 8. Adsorption of a 0.01 mg/mL solution of mAb-1 to an OTS-coated glass surface pre-coated with polysorbate in histidine buffer pH 5.5, investigated using TIRF, as follows: Tween 20, 0.05 mM (red line) and 1 mM (gray line); Tween 80, 5 µM (blue line) and 1 mM (green line). Referenced to the adsorption of a 0.01 mg/mL solution of mAb-1 to a OTS-coated glass surface (black line).