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Figure 1. Affinity determination of the CRP specific scFv antibodies by SPR analysis. CRP was covalently coupled to a CM5 sensor chip. Sensorgrams of the CRP specific scFv antibody clones LA13-IIE3 (A), LA13-IIC3 (B) and LA13-IID4 (C) are shown. Four to five scFv concentrations were used to determine the affinity constants KD and off rates koff (D).
