Figure 2. Size-exclusion chromatography of affinity-purified scFv LA13-IIE3 on a calibrated Superdex 200 10/300 GL column. Molar mass calibration (closed orange squares) was done with blue dextran (2,000 kDa), β-amylase (200 kDa), alcohol dehydrogenase (150 kDa), albumin (66 kDa), carbonic anhydrase (29 kDa) and cytochrome C (12.4 kDa).