Figure 6. Human T cell activation studies. (A) FACS analysis of CD69 expression by Jurkat cells stimulated either with soluble anti-CD3 (OKT3) or anti-MHC class I (W6/32) mAb, with conditioned media from HEK-293 cells (293-CM) transfected with expression vectors encoding scFv-based trimerbodies (L36^N, L36^N/CL36, ^COKT3 or L36^N/COKT3), or with purified trimerbodies (L36N or L36^N/COKT3). (B) FACS analysis of CD69 expression by Jurkat cells stimulated either with plastic immobilized mAb (imAb), BSA (iBSA) or LM-111 (iLM-111) in the presence of 293-CM from L36^N, L36^N/CL36, ^COKT3 or L36^N/COKT3 transfected cells or purified L36^N or L36^N/COKT3 trimerbodies.