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. 1972 Sep;10(3):484–491. doi: 10.1128/jvi.10.3.484-491.1972

Origin and Direction of Simian Virus 40 Deoxyribonucleic Acid Replication

George C Fareed 1, Claude F Garon 1, Norman P Salzman 1
PMCID: PMC356490  PMID: 4342055

Abstract

Double-branched, circular, replicating deoxyribonucleic acid (DNA) molecules of simian virus 40 (SV40) have been cleaved by the R1 restriction endonuclease from Escherichia coli. This enzyme introduces one double-strand break in SV40 DNA, at a specific site. The site of cleavage in the replicating molecules was used in this study to position the origin and the two branch points. Radioactively labeled molecules fractionated according to their extent of replication were evaluated after cleavage by sedimentation analysis and electron microscopy. The results demonstrate that the R1 cleavage site is 33% of the genome length from the origin of replication and that both branch points are growing points. These data indicate that SV40 DNA replication is bidirectional and confirm other reports which have shown a unique origin of replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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