Abstract
Some temperature-sensitive mutants of vesicular stomatitis virus were tested for their ability to block the initiation of deoxyribonucleic acid (DNA) synthesis and division in serum-stimulated hamster embryo fibroblasts at the nonpermissive temperature. Although the parental strain blocked these processes, one particular mutant allowed essentially normal DNA synthesis and division. By autoradiography, it was shown that individual cells infected with this mutant could synthesize viral ribonucleic acid and at the same time initiate DNA synthesis and divide. Cells infected with such conditional defective mutants appear to be suitable for studies on the effects of persistent viral infections on molecular and cellular functions in proliferating cell populations.
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- Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
- DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ehrenfeld E., Hunt T. Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte lysates. Proc Natl Acad Sci U S A. 1971 May;68(5):1075–1078. doi: 10.1073/pnas.68.5.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gajdusek D. C. Slow virus diseases of the central nervous system. Am J Clin Pathol. 1971 Sep;56(3):320–332. doi: 10.1093/ajcp/56.3.320. [DOI] [PubMed] [Google Scholar]
- Howatson A. F. Vesicular stomatitis and related viruses. Adv Virus Res. 1970;16:195–256. doi: 10.1016/s0065-3527(08)60024-x. [DOI] [PubMed] [Google Scholar]
- STANNERS C. P., TILL J. E. DNA synthesis in individual L-strain mouse cells. Biochim Biophys Acta. 1960 Jan 29;37:406–419. doi: 10.1016/0006-3002(60)90496-0. [DOI] [PubMed] [Google Scholar]
- Schincariol A. L., Howatson A. F. Replication of vesicular stomatitis virus. I. Viral specific RNA and nucleoprotein in infected L cells. Virology. 1970 Nov;42(3):732–743. doi: 10.1016/0042-6822(70)90319-3. [DOI] [PubMed] [Google Scholar]
- Stanners C. P., Becker H. Control of macromolecular synthesis in proliferating and resting Syrian hamster cells in monolayer culture. I. Ribosome function. J Cell Physiol. 1971 Feb;77(1):31–42. doi: 10.1002/jcp.1040770105. [DOI] [PubMed] [Google Scholar]
- Stanners C. P., Eliceiri G. L., Green H. Two types of ribosome in mouse-hamster hybrid cells. Nat New Biol. 1971 Mar 10;230(10):52–54. doi: 10.1038/newbio230052a0. [DOI] [PubMed] [Google Scholar]
- Suskind R. G., Pry T. W., Rabotti G. F. Effect of Rous sarcoma virus infection on recovery of nucleolar RNA, ribonucleoprotein, and DNA synthesis from inhibition by actinomycin D. Cancer Res. 1969 Aug;29(8):1598–1605. [PubMed] [Google Scholar]
- Todaro G., Matsuya Y., Bloom S., Robbins A., Green H. Stimulation of RNA synthesis and cell division in resting cells by a factor present in serum. Wistar Inst Symp Monogr. 1967;7:87–101. [PubMed] [Google Scholar]
- Wertz G. W., Youngner J. S. Inhibition of protein synthesis in L cells infected with vesicular stomatitis virus. J Virol. 1972 Jan;9(1):85–89. doi: 10.1128/jvi.9.1.85-89.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yaoi Y., Amano M. Inhibitory effect of ultraviolet-inactivated vesicular stomatitis virus on initiation o DNA synthesis in cultured chick embryo cells. J Gen Virol. 1970 Oct;9(1):69–75. doi: 10.1099/0022-1317-9-1-69. [DOI] [PubMed] [Google Scholar]
- Yaoi Y., Mitsui H., Amano M. Effect of U.v.-irradiated vesicular stomatitis virus on nucleic acid synthesis in chick embryo cells. J Gen Virol. 1970 Sep;8(3):165–172. doi: 10.1099/0022-1317-8-3-165. [DOI] [PubMed] [Google Scholar]