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. 1972 Oct;10(4):653–657. doi: 10.1128/jvi.10.4.653-657.1972

Isolation of Temperature-Sensitive Mutants of Murine Sarcoma Virus

Edward M Scolnick 1, John R Stephenson 1, Stuart A Aaronson 1
PMCID: PMC356516  PMID: 4117345

Abstract

Three separate murine sarcoma virus nonproducer cell lines have been isolated which are temperature sensitive for the maintenance of transformation. In each case, a viral rather than a cellular genetic mutation is the reason for the temperature-sensitive effect. Superinfection of one of the mutants with murine leukemia virus overcomes the temperature-sensitive change in the transformed state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Rowe S. P. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology. 1970 Sep;42(1):9–19. doi: 10.1016/0042-6822(70)90233-3. [DOI] [PubMed] [Google Scholar]
  2. Aaronson S. A., Todaro G. J. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J Cell Physiol. 1968 Oct;72(2):141–148. doi: 10.1002/jcp.1040720208. [DOI] [PubMed] [Google Scholar]
  3. Aaronson S. A., Weaver C. A. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J Gen Virol. 1971 Nov;13(2):245–252. doi: 10.1099/0022-1317-13-2-245. [DOI] [PubMed] [Google Scholar]
  4. Ando T. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae. Biochim Biophys Acta. 1966 Jan 18;114(1):158–168. doi: 10.1016/0005-2787(66)90263-2. [DOI] [PubMed] [Google Scholar]
  5. Bader J. P., Brown N. R. Induction of mutations in an RNA tumour virus by an analogue of a DNA precursor. Nat New Biol. 1971 Nov 3;234(44):11–12. doi: 10.1038/newbio234011a0. [DOI] [PubMed] [Google Scholar]
  6. Biquard J. M., Vigier P. Characteristics of a conditional mutant of Rous sarcoma virus defective in ability to transform cells at high temperature. Virology. 1972 Feb;47(2):444–455. doi: 10.1016/0042-6822(72)90280-2. [DOI] [PubMed] [Google Scholar]
  7. Friis R. R., Toyoshima K., Vogt P. K. Conditional lethal mutants of avian sarcoma viruses. I. Physiology of ts 75 and ts 149. Virology. 1971 Feb;43(2):375–389. doi: 10.1016/0042-6822(71)90310-2. [DOI] [PubMed] [Google Scholar]
  8. GROSS L. Viral etiology of spontaneous mouse leukemia; a review. Cancer Res. 1958 May;18(4):371–381. [PubMed] [Google Scholar]
  9. Green M., Rokutanda H., Rokutanda M. Virus specific RNA in cells transformed by RNA tumour viruses. Nat New Biol. 1971 Apr 21;230(16):229–232. doi: 10.1038/newbio230229a0. [DOI] [PubMed] [Google Scholar]
  10. Hartley J. W., Rowe W. P. Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proc Natl Acad Sci U S A. 1966 Apr;55(4):780–786. doi: 10.1073/pnas.55.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1164–1169. doi: 10.1073/pnas.56.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Katz E., Vogt P. K. Conditional lethal mutants of avian sarcoma viruses. II. Analysis of the temperature-sensitive lesion in ts 75. Virology. 1971 Dec;46(3):745–753. doi: 10.1016/0042-6822(71)90076-6. [DOI] [PubMed] [Google Scholar]
  13. Kawai S., Hanafusa H. Plaque assay for some strains of avian leukosis virus. Virology. 1972 Apr;48(1):126–135. doi: 10.1016/0042-6822(72)90120-1. [DOI] [PubMed] [Google Scholar]
  14. Kawai S., Hanafusa H. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant. Virology. 1971 Nov;46(2):470–479. doi: 10.1016/0042-6822(71)90047-x. [DOI] [PubMed] [Google Scholar]
  15. Martin G. S. Rous sarcoma virus: a function required for the maintenance of the transformed state. Nature. 1970 Sep 5;227(5262):1021–1023. doi: 10.1038/2271021a0. [DOI] [PubMed] [Google Scholar]
  16. Moloney J. B. A virus-induced rhabdomyosarcoma of mice. Natl Cancer Inst Monogr. 1966 Sep;22:139–142. [PubMed] [Google Scholar]
  17. Pollack R. E., Green H., Todaro G. J. Growth control in cultured cells: selection of sublines with increased sensitivity to contact inhibition and decreased tumor-producing ability. Proc Natl Acad Sci U S A. 1968 May;60(1):126–133. doi: 10.1073/pnas.60.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Renger H. C., Basilico C. Mutation causing temperature-sensitive expression of cell transformation by a tumor virus (SV40-3T3 mouse cells-growth control). Proc Natl Acad Sci U S A. 1972 Jan;69(1):109–114. doi: 10.1073/pnas.69.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  20. Stephenson J. R., Reynolds R. K., Aaronson S. A. Isolation of temperature-sensitive mutants of murine leukemia virus. Virology. 1972 Jun;48(3):749–756. doi: 10.1016/0042-6822(72)90158-4. [DOI] [PubMed] [Google Scholar]
  21. Stephenson J. R., Scolnick E. M., Aaronson S. A. Genetic stability of the sarcoma viruses in murine and avian sarcoma virus-transformed nonproducer cells. Int J Cancer. 1972 May 15;9(3):577–583. doi: 10.1002/ijc.2910090314. [DOI] [PubMed] [Google Scholar]
  22. Toyoshima K., Vogt P. K. Temperature sensitive mutants of an avian sarcoma virus. Virology. 1969 Dec;39(4):930–931. doi: 10.1016/0042-6822(69)90030-0. [DOI] [PubMed] [Google Scholar]

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